yamada1

The SBML for this model was obtained from the BioModels database (BioModels ID: BIOMD0000000093). Biomodels notes: "NCBS Curation Comments This model shows the control mechanism of Jak-Stat pathway, here SOCS1 (Suppressor of cytokine signaling-I) was identified as the negative regulator of Jak and STAT signal transduction pathway. Note: There are a few ambiguities in the paper like initial concentration of IFN and some reactions were missing in the paper that were employed for obtaining the results. The graphs are almost similar to the graphs as shown in the paper but still some ambiguities regarding the concentration are there. Thanks to Dr Satoshi Yamada for clarifying some of those ambiguities and providing the values used in simulations. Biomodels Curation Comments The model reproduces Fig 2 (A,C,E,G,I,K,M) of the paper. The set of equations present in the paper are inadequate to reproduce the figures mentioned . The model appears to have been fine tuned after correspondence between the curators at NCBS and the authors. There is however a slight discrepancy between the simulation results and the plots in the paper. The model was tested on MathSBML." JWS Online curation: This model was curated by reproducing the figures as described in the BioModels Notes. No additional changes were made.

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Control mechanism of JAK/STAT signal transduction pathway.

  • Satoshi Yamada
  • Satoru Shiono
  • Akiko Joo
  • Akihiko Yoshimura
FEBS Lett. 2003; 534 : 190-196
Abstract
Suppressor of cytokine signaling-1 (SOCS1) was identified as the negative regulator of Janus kinase (JAK) and signal transducer and activator of transcription (STAT) signal transduction pathway. However, the kinetics and control mechanism of the pathway have not yet been fully understood. We have developed the computer simulation of the JAK/STAT pathway. Without nuclear phosphatase, SOCS1's binding to JAK did not cause the decrease in nuclear phosphorylated STAT1. However, without SH2 domain-containing tyrosine phosphatase 2 (SHP-2) or cytoplasmic phosphatase, it did. So nuclear phosphatase is considered to be the most important in this system. By changing parameters of the model, dynamical characteristics and control mechanism were investigated.

Unit definitions have no effect on the numerical analysis of the model. It remains the responsibility of the modeler to ensure the internal numerical consistency of the model. If units are provided, however, the consistency of the model units will be checked.

Name Definition
1e-09 mole
1e-09 mole litre^(-1.0) second^(-1.0)
1.0 second^(-1.0)
1000000000.0 mole^(-1.0) litre second^(-1.0)
1e-09 mole litre^(-1.0)
Id Name Spatial dimensions Size
cytoplasm cytoplasm 3.0 1.0
nucleus nucleus 3.0 1.0
Id Name Initial quantity Compartment Fixed
IFN IFN 10.0 cytoplasm (cytoplasm)
IFNR IFNR 0.0 cytoplasm (cytoplasm)
IFNRJ Interferon-Receptor-JAK complex 0.0 cytoplasm (cytoplasm)
IFNRJ2 IFNRJ dimer 0.0 cytoplasm (cytoplasm)
IFNRJ2_star Activated IFNRJ complex 0.0 cytoplasm (cytoplasm)
IFNRJ2_star_SHP2 IFNRJ2_star_SHP2 0.0 cytoplasm (cytoplasm)
IFNRJ2_star_SHP2_SOCS1 IFNRJ2_star_SHP2_SOCS1 0.0 cytoplasm (cytoplasm)
IFNRJ2_star_SHP2_SOCS1_STAT1c IFNRJ2_star_SHP2_SOCS1_STAT1c 0.0 cytoplasm (cytoplasm)
IFNRJ2_star_SHP2_STAT1c IFNRJ2_star_SHP2_STAT1c 0.0 cytoplasm (cytoplasm)
IFNRJ2_star_SOCS1 IFNRJ2_star_SOCS1 0.0 cytoplasm (cytoplasm)
IFNRJ2_star_SOCS1_STAT1c IFNRJ2_star_SOCS1_STAT1c 0.0 cytoplasm (cytoplasm)
IFNRJ2_star_STAT1c IFNRJ2_star_STAT1c 0.0 cytoplasm (cytoplasm)
IFNRJ2_star_STAT1c_star IFNRJ2_star_STAT1c_star 0.0 cytoplasm (cytoplasm)
JAK JAK 10.0 cytoplasm (cytoplasm)
PPN PPN 60.0 nucleus (nucleus)
PPX PPX 50.0 cytoplasm (cytoplasm)
R Receptor 10.0 cytoplasm (cytoplasm)
RJ Receptor JAK complex 0.0 cytoplasm (cytoplasm)
SHP2 SHP2 100.0 cytoplasm (cytoplasm)
SOCS1 SOCS1 0.0 cytoplasm (cytoplasm)
STAT1c STAT1c 1000.0 cytoplasm (cytoplasm)
STAT1c_STAT1c_star STAT1c_STAT1c_star 0.0 cytoplasm (cytoplasm)
STAT1c_star STAT1c_star 0.0 cytoplasm (cytoplasm)
STAT1c_star_PPX STAT1c_star_PPX 0.0 cytoplasm (cytoplasm)
STAT1c_star_STAT1c_star STAT1c_star_STAT1c_star 0.0 cytoplasm (cytoplasm)
STAT1c_star_STAT1c_star_PPX STAT1c_star_STAT1c_star_PPX 0.0 cytoplasm (cytoplasm)
STAT1n STAT1n 0.0 nucleus (nucleus)
STAT1n_STAT1n_star STAT1n_STAT1n_star 0.0 nucleus (nucleus)
STAT1n_star STAT1n_star 0.0 nucleus (nucleus)
STAT1n_star_PPN STAT1n_star_PPN 0.0 nucleus (nucleus)
STAT1n_star_STAT1n_star STAT1n_star_STAT1n_star 0.0 nucleus (nucleus)
STAT1n_star_STAT1n_star_PPN STAT1n_star_STAT1n_star_PPN 0.0 nucleus (nucleus)
mRNAc mRNAc 0.0 cytoplasm (cytoplasm)
mRNAn mRNAn 0.0 nucleus (nucleus)

Initial assignments are expressions that are evaluated at time=0. It is not recommended to create initial assignments for all model entities. Restrict the use of initial assignments to cases where a value is expressed in terms of values or sizes of other model entities. Note that it is not permitted to have both an initial assignment and an assignment rule for a single model entity.

Definition
Id Name Objective coefficient Reaction Equation and Kinetic Law Flux bounds
v1 Receptor-JAK Binding JAK + R > RJ

cytoplasm * (kf * R * JAK - kb * RJ)
v10 IFNJR2 dephosphorylation IFNRJ2_star_SHP2 > SHP2 + IFNRJ2

cytoplasm * kf * IFNRJ2_star_SHP2
v11 Phosphorylated STAT1c-PPX binding STAT1c_star + PPX > STAT1c_star_PPX

cytoplasm * (kf * PPX * STAT1c_star - kb * STAT1c_star_PPX)
v12 STAT1c dephosphorylation STAT1c_star_PPX > STAT1c + PPX

cytoplasm * kf * STAT1c_star_PPX
v13 PPX binding STAT1c_star_STAT1c_star + PPX > STAT1c_star_STAT1c_star_PPX

cytoplasm * (kf * PPX * STAT1c_star_STAT1c_star - kb * STAT1c_star_STAT1c_star_PPX)
v14 STAT1c dimer dephosphorylation STAT1c_star_STAT1c_star_PPX > STAT1c_STAT1c_star + PPX

cytoplasm * kf * STAT1c_star_STAT1c_star_PPX
v15 STAT1c-phosphorylated STAT1c binding STAT1c_star + STAT1c > STAT1c_STAT1c_star

cytoplasm * (kf * STAT1c * STAT1c_star - kb * STAT1c_STAT1c_star)
v16 STAT1c-nuclear transport STAT1c_star_STAT1c_star > STAT1n_star_STAT1n_star

cytoplasm * kf * STAT1c_star_STAT1c_star
v17 Phosphorylated STAT1n dimerization {2.0}STAT1n_star > STAT1n_star_STAT1n_star

nucleus * (kf * STAT1n_star * STAT1n_star - kb * STAT1n_star_STAT1n_star)
v18 PPN binding STAT1n_star + PPN > STAT1n_star_PPN

nucleus * (kf * PPN * STAT1n_star - kb * STAT1n_star_PPN)
v19 STAT1n dephosphorylation STAT1n_star_PPN > STAT1n + PPN

nucleus * kf * STAT1n_star_PPN
v2 Interferon-Receptor Binding RJ + IFN > IFNRJ

cytoplasm * (kf * IFN * RJ - kb * IFNRJ)
v20 PPN binding STAT1n_star_STAT1n_star + PPN > STAT1n_star_STAT1n_star_PPN

nucleus * (kf * PPN * STAT1n_star_STAT1n_star - kb * STAT1n_star_STAT1n_star_PPN)
v21 STAT1n dephosphorylation STAT1n_star_STAT1n_star_PPN > STAT1n_STAT1n_star + PPN

nucleus * kf * STAT1n_star_STAT1n_star_PPN
v22 STAT1n-phosphorylated STAT1n dimerization STAT1n_star + STAT1n > STAT1n_STAT1n_star

nucleus * (kf * STAT1n * STAT1n_star - kb * STAT1n_STAT1n_star)
v23 STAT1n transport to cytoplasm STAT1n > STAT1c

nucleus * kf * STAT1n
v24 Transcription ∅ > mRNAn

nucleus * ka * STAT1n_star_STAT1n_star / (kb + STAT1n_star_STAT1n_star)
v25 mRNA transport to cytoplasm mRNAn > mRNAc

nucleus * kf * mRNAn
v26 SOCS1 synthesis ∅ > SOCS1

cytoplasm * kf * mRNAc
v27 mRNAc degradation mRNAc > ∅

cytoplasm * kf * mRNAc
v28 SOCS1 degradation SOCS1 > ∅

cytoplasm * kf * SOCS1
v29 phosphorylated IFNRJ2-SOCS1 binding IFNRJ2_star + SOCS1 > IFNRJ2_star_SOCS1

cytoplasm * (kf * SOCS1 * IFNRJ2_star - kb * IFNRJ2_star_SOCS1)
v3 IFN-Receptor complex dimerization {2.0}IFNRJ > IFNRJ2

cytoplasm * (kf * IFNRJ * IFNRJ - kb * IFNRJ2)
v30 STAT1c binding IFNRJ2_star_SOCS1 + STAT1c > IFNRJ2_star_SOCS1_STAT1c

cytoplasm * (kf * STAT1c * IFNRJ2_star_SOCS1 - kb * IFNRJ2_star_SOCS1_STAT1c)
v31 SHP2 binding IFNRJ2_star_SOCS1_STAT1c + SHP2 > IFNRJ2_star_SHP2_SOCS1_STAT1c

cytoplasm * (kf * SHP2 * IFNRJ2_star_SOCS1_STAT1c - kb * IFNRJ2_star_SHP2_SOCS1_STAT1c)
v32 IFNRJ2 dephosphorylation IFNRJ2_star_SHP2_SOCS1_STAT1c > IFNRJ2 + SOCS1 + STAT1c + SHP2

cytoplasm * kf * IFNRJ2_star_SHP2_SOCS1_STAT1c
v33 SOCS1 unbinding IFNRJ2_star_SHP2_SOCS1_STAT1c > IFNRJ2_star_SHP2_STAT1c

cytoplasm * kf * IFNRJ2_star_SHP2_SOCS1_STAT1c
v34 SHP2 binding IFNRJ2_star_SOCS1 + SHP2 > IFNRJ2_star_SHP2_SOCS1

cytoplasm * (kf * SHP2 * IFNRJ2_star_SOCS1 - kb * IFNRJ2_star_SHP2_SOCS1)
v35 STAT1c binding IFNRJ2_star_SHP2_SOCS1 + STAT1c > IFNRJ2_star_SHP2_SOCS1_STAT1c

cytoplasm * (kf * STAT1c * IFNRJ2_star_SHP2_SOCS1 - kb * IFNRJ2_star_SHP2_SOCS1_STAT1c)
v36 SHP2 binding IFNRJ2_star_STAT1c + SHP2 > IFNRJ2_star_SHP2_STAT1c

cytoplasm * (kf * SHP2 * IFNRJ2_star_STAT1c - kb * IFNRJ2_star_SHP2_STAT1c)
v37 IFNRJ2_star_SHP2_STAT1c > STAT1c + SHP2 + IFNRJ2

cytoplasm * kf * IFNRJ2_star_SHP2_STAT1c
v38 SOCS1 unbinding IFNRJ2_star_SOCS1_STAT1c > IFNRJ2_star_STAT1c

cytoplasm * kf * IFNRJ2_star_SOCS1_STAT1c
v39 SOCS1 unbinding IFNRJ2_star_SHP2_SOCS1 > IFNRJ2_star_SHP2

cytoplasm * kf * IFNRJ2_star_SHP2_SOCS1
v4 INF-Receptor complex activation IFNRJ2 > IFNRJ2_star

cytoplasm * kf * IFNRJ2
v40 IFNRJ2 dephosphorylation IFNRJ2_star_SHP2_SOCS1 > SHP2 + IFNRJ2 + SOCS1

cytoplasm * kf * IFNRJ2_star_SHP2_SOCS1
v41 SOCS1 unbinding IFNRJ2_star_SOCS1 > IFNRJ2_star

cytoplasm * kf * IFNRJ2_star_SOCS1
v42 SOCS1 binding IFNRJ2_star_STAT1c + SOCS1 > IFNRJ2_star_SOCS1_STAT1c

cytoplasm * (kf * SOCS1 * IFNRJ2_star_STAT1c - kb * IFNRJ2_star_SOCS1_STAT1c)
v43 SOCS1 binding IFNRJ2_star_SHP2 + SOCS1 > IFNRJ2_star_SHP2_SOCS1

cytoplasm * (kf * SOCS1 * IFNRJ2_star_SHP2 - kb * IFNRJ2_star_SHP2_SOCS1)
v44 SOCS1 binding IFNRJ2_star_SHP2_STAT1c + SOCS1 > IFNRJ2_star_SHP2_SOCS1_STAT1c

cytoplasm * (kf * SOCS1 * IFNRJ2_star_SHP2_STAT1c - kb * IFNRJ2_star_SHP2_SOCS1_STAT1c)
v45 Interferon-receptor binding R + IFN > IFNR

cytoplasm * (kf * IFN * R - kb * IFNR)
v46 IFNR-JAK binding JAK + IFNR > IFNRJ

cytoplasm * (kf * IFNR * JAK - kb * IFNRJ)
v5 Activated INFRJ2-STAT1c binding IFNRJ2_star + STAT1c > IFNRJ2_star_STAT1c

cytoplasm * (kf * STAT1c * IFNRJ2_star - kb * IFNRJ2_star_STAT1c)
v6 STAT1c activation IFNRJ2_star_STAT1c > STAT1c_star + IFNRJ2_star

cytoplasm * kf * IFNRJ2_star_STAT1c
v7 Activated IFNRJ2-STAT1c binding STAT1c_star + IFNRJ2_star > IFNRJ2_star_STAT1c_star

cytoplasm * (kf * IFNRJ2_star * STAT1c_star - kb * IFNRJ2_star_STAT1c_star)
v8 Activated STAT1c dimerization {2.0}STAT1c_star > STAT1c_star_STAT1c_star

cytoplasm * (kf * STAT1c_star * STAT1c_star - kb * STAT1c_star_STAT1c_star)
v9 SHP2 binding SHP2 + IFNRJ2_star > IFNRJ2_star_SHP2

cytoplasm * (kf * IFNRJ2_star * SHP2 - kb * IFNRJ2_star_SHP2)

Global parameters

Id Value

Local parameters

Id Value Reaction
kf 0.1 nM_inv_sec_inv v1 (Receptor-JAK Binding)
kb 0.05 sec_inv v1 (Receptor-JAK Binding)
kf 0.02 nM_inv_sec_inv v2 (Interferon-Receptor Binding)
kb 0.02 sec_inv v2 (Interferon-Receptor Binding)
kf 0.04 nM_inv_sec_inv v3 (IFN-Receptor complex dimerization)
kb 0.2 sec_inv v3 (IFN-Receptor complex dimerization)
kf 0.005 sec_inv v4 (INF-Receptor complex activation)
kf 0.008 nM_inv_sec_inv v5 (Activated INFRJ2-STAT1c binding)
kb 0.8 sec_inv v5 (Activated INFRJ2-STAT1c binding)
kf 0.4 sec_inv v6 (STAT1c activation)
kf 0.005 nM_inv_sec_inv v7 (Activated IFNRJ2-STAT1c binding)
kb 0.5 sec_inv v7 (Activated IFNRJ2-STAT1c binding)
kf 0.02 nM_inv_sec_inv v8 (Activated STAT1c dimerization)
kb 0.1 sec_inv v8 (Activated STAT1c dimerization)
kf 0.001 nM_inv_sec_inv v9 (SHP2 binding)
kb 0.2 sec_inv v9 (SHP2 binding)
kf 0.003 sec_inv v10 (IFNJR2 dephosphorylation)
kf 0.001 nM_inv_sec_inv v11 (Phosphorylated STAT1c-PPX binding)
kb 0.2 sec_inv v11 (Phosphorylated STAT1c-PPX binding)
kf 0.003 sec_inv v12 (STAT1c dephosphorylation)
kf 0.001 nM_inv_sec_inv v13 (PPX binding)
kb 0.2 sec_inv v13 (PPX binding)
kf 0.003 sec_inv v14 (STAT1c dimer dephosphorylation)
kf 2e-07 nM_inv_sec_inv v15 (STAT1c-phosphorylated STAT1c binding)
kb 0.2 sec_inv v15 (STAT1c-phosphorylated STAT1c binding)
kf 0.005 sec_inv v16 (STAT1c-nuclear transport)
kf 0.02 nM_inv_sec_inv v17 (Phosphorylated STAT1n dimerization)
kb 0.1 sec_inv v17 (Phosphorylated STAT1n dimerization)
kf 0.001 nM_inv_sec_inv v18 (PPN binding)
kb 0.2 sec_inv v18 (PPN binding)
kf 0.005 sec_inv v19 (STAT1n dephosphorylation)
kf 0.001 nM_inv_sec_inv v20 (PPN binding)
kb 0.2 sec_inv v20 (PPN binding)
kf 0.005 sec_inv v21 (STAT1n dephosphorylation)
kf 2e-07 nM_inv_sec_inv v22 (STAT1n-phosphorylated STAT1n dimerization)
kb 0.2 sec_inv v22 (STAT1n-phosphorylated STAT1n dimerization)
kf 0.05 sec_inv v23 (STAT1n transport to cytoplasm)
ka 0.01 nM_per_second v24 (Transcription)
kb 400.0 nM v24 (Transcription)
kf 0.001 sec_inv v25 (mRNA transport to cytoplasm)
kf 0.01 sec_inv v26 (SOCS1 synthesis)
kf 0.0005 sec_inv v27 (mRNAc degradation)
kf 0.0005 sec_inv v28 (SOCS1 degradation)
kf 0.02 nM_inv_sec_inv v29 (phosphorylated IFNRJ2-SOCS1 binding)
kb 0.1 sec_inv v29 (phosphorylated IFNRJ2-SOCS1 binding)
kf 0.008 nM_inv_sec_inv v30 (STAT1c binding)
kb 0.8 sec_inv v30 (STAT1c binding)
kf 0.001 nM_inv_sec_inv v31 (SHP2 binding)
kb 0.2 sec_inv v31 (SHP2 binding)
kf 0.003 sec_inv v32 (IFNRJ2 dephosphorylation)
kf 0.0005 sec_inv v33 (SOCS1 unbinding)
kf 0.001 nM_inv_sec_inv v34 (SHP2 binding)
kb 0.2 sec_inv v34 (SHP2 binding)
kf 0.008 nM_inv_sec_inv v35 (STAT1c binding)
kb 0.8 sec_inv v35 (STAT1c binding)
kf 0.001 nM_inv_sec_inv v36 (SHP2 binding)
kb 0.2 sec_inv v36 (SHP2 binding)
kf 0.003 sec_inv v37
kf 0.0005 sec_inv v38 (SOCS1 unbinding)
kf 0.0005 sec_inv v39 (SOCS1 unbinding)
kf 0.003 sec_inv v40 (IFNRJ2 dephosphorylation)
kf 0.0005 sec_inv v41 (SOCS1 unbinding)
kf 0.02 nM_inv_sec_inv v42 (SOCS1 binding)
kb 0.1 sec_inv v42 (SOCS1 binding)
kf 0.02 nM_inv_sec_inv v43 (SOCS1 binding)
kb 0.1 sec_inv v43 (SOCS1 binding)
kf 0.02 nM_inv_sec_inv v44 (SOCS1 binding)
kb 0.1 sec_inv v44 (SOCS1 binding)
kf 0.02 nM_inv_sec_inv v45 (Interferon-receptor binding)
kb 0.02 sec_inv v45 (Interferon-receptor binding)
kf 0.1 nM_inv_sec_inv v46 (IFNR-JAK binding)
kb 0.05 sec_inv v46 (IFNR-JAK binding)

Assignment rules

Definition

Rate rules

Definition

Algebraic rules

Definition
Trigger Assignments