begin name pritchart1 end name begin reactions Calloseproduction {1.0}PRR = {1.0}PRR + {1.0}Callose Calloseremoval {1.0}Callose = {1.0}$EXTERNAL Callosesuppression {1.0}Callose + {1.0}Eint = {1.0}Eint ETI {1.0}Path + {1.0}R0 = {1.0}R0 Eintremoval {1.0}Eint = {1.0}$EXTERNAL Effectorproduction {1.0}Path = {1.0}E + {1.0}Path Effectorrecognition {1.0}R + {1.0}Eint = {1.0}R0 Effectorremoval {1.0}E = {1.0}$EXTERNAL Effectortranslocation {1.0}E = {1.0}Eint PAMPproduction {1.0}Path = {1.0}PAMP + {1.0}Path PAMPrecognition {1.0}PRR0 + {1.0}PAMP = {1.0}PRR PAMPremoval {1.0}PAMP = {1.0}$EXTERNAL PTI {1.0}Path + {1.0}Callose = {1.0}Callose Pathogenarrival {1.0}Pathbulk = {1.0}Pathbulk + {1.0}Path Pathogenremoval {1.0}Path = {1.0}$EXTERNAL end reactions begin rate equations Calloseproduction = Calloseproductionk1*PRR[t] Calloseremoval = Calloseremovalk1*Cell*Callose[t] Callosesuppression = Callosesuppressionk1*Cell*Callose[t]*Eint[t] ETI = ETIk1*Path[t]*R0[t] Eintremoval = Cell*Eintremovalk1*Eint[t] Effectorproduction = Apoplast*Effectorproductionk1*Path[t] Effectorrecognition = Cell*(Effectorrecognitionk1*Eint[t]*R[t] - Effectorrecognitionk2*R0[t]) Effectorremoval = Apoplast*Effectorremovalk1*E[t] Effectortranslocation = Competitiveinhibitionirr[E[t], Callose[t], EffectortranslocationKm, EffectortranslocationV, EffectortranslocationKi] PAMPproduction = PAMPproductionk1*Path[t] PAMPrecognition = -(PAMPrecognitionk2*PRR[t]) + PAMPrecognitionk1*PAMP[t]*PRR0[t] PAMPremoval = Cell*PAMPremovalk1*PAMP[t] PTI = PTIk1*Callose[t]*Path[t] Pathogenarrival = Apoplast*Pathogenarrivalk1*Pathbulk[t] Pathogenremoval = Apoplast*Pathogenremovalk1*Path[t] end rate equations begin parameters PAMPrecognitionk1 = 0.1 PAMPrecognitionk2 = 0.1 Effectorrecognitionk1 = 0.1 Effectorrecognitionk2 = 0.1 Effectorremovalk1 = 0.1 PAMPremovalk1 = 0.1 Pathogenarrivalk1 = 0.1 Pathogenremovalk1 = 0.1 PAMPproductionk1 = 0.1 Effectorproductionk1 = 0.1 Eintremovalk1 = 0.1 ETIk1 = 0.1 EffectortranslocationKm = 0.1 EffectortranslocationV = 0.1 EffectortranslocationKi = 0.1 Calloseproductionk1 = 0.1 Calloseremovalk1 = 0.1 PTIk1 = 0.1 Callosesuppressionk1 = 0.1 Apoplast = 1.0 Cell = 1.0 end parameters begin initial conditions Callose[0] = Callosei E[0] = Ei Eint[0] = Einti PAMP[0] = PAMPi PRR[0] = PRRi PRR0[0] = PRR0i Path[0] = Pathi Pathbulk[0] = Pathbulki R[0] = Ri R0[0] = R0i end initial conditions begin initial values Callosei = 0.0 Ei = 0.0 Einti = 0.0 PAMPi = 0.0 PRRi = 0.0 PRR0i = 1.0 Pathi = 0.0 Pathbulki = 0.0 Ri = 1.0 R0i = 0.0 end initial values begin assignment rules end assignment rules begin function definitions Competitiveinhibitionirr[substrate_,Inhibitor_,Km_,V_,Ki_] := (substrate*V)/(Km + (Inhibitor*Km)/Ki + substrate) end function definitions begin events Pathogen_introduced = (t > 10, {Pathbulk[t] -> 1}, 0) end events begin process annotations end process annotations begin species annotations end species annotations begin units end units