begin name
nazaret1
end name

begin reactions
v1 {1.0}$EXTERNAL = {1.0}Pyr
v2 {1.0}Pyr + {1.0}NAD = {1.0}AcCoA
v3 {1.0}OAA + {1.0}AcCoA = {1.0}Cit
v4 {1.0}Cit + {1.0}NAD = {1.0}KG
v5 {1.0}KG + {2.0}NAD = {1.0}OAA + {1.0}ATP
v6 {1.0}OAA = {1.0}KG
v7 {1.0}Pyr + {1.0}ATP = {1.0}OAA
v8 {1.0}OAA = {1.0}$EXTERNAL
vANT {1.0}ATP = {1.0}$EXTERNAL
vATP {1.0}$iP + {3.0}$He = {1.0}ATP + {1.0}$H2O + {3.0}$H
vleak {1.0}$He = {1.0}$H
vresp {0.5}$O2 + {11.0}$H = {1.0}NAD + {1.0}$H2O + {10.0}$He
end reactions

begin rate equations
v1 = mitochondrion*v1k1
v2 = mitochondrion*v2k2*NAD[t]*Pyr[t]
v3 = mitochondrion*v3k3*AcCoA[t]*OAA[t]
v4 = mitochondrion*v4k4*Cit[t]*NAD[t]
v5 = mitochondrion*v5k5*(At - ATP[t])*KG[t]*NAD[t]
v6 = mitochondrion*v6k6*(-(KG[t]/v6Keq) + OAA[t])
v7 = mitochondrion*v7k7*ATP[t]*Pyr[t]
v8 = mitochondrion*v8k8*OAA[t]
vANT = JANT*mitochondrion
vATP = JATP*mitochondrion
vleak = Jleak*mitochondrion
vresp = Jresp*mitochondrion
end rate equations

begin parameters
At = 4.16
C = "6.75e-06"
DeltaPsim = 150.0
F = 96485.0
K = 2.0
Kapp = "4.4e-09"
Nt = 1.07
R = 8314.0
T = 298.0
a = 0.1
b = 0.004
kANT = 0.05387
kATP = 131.9
kleak = 0.000426
kresp = 2.5
H = 1.0
H2O = 1.0
He = 1.0
O2 = 1.0
iP = 2.44
v1k1 = 0.038
v2k2 = 0.152
v3k3 = 57.142
v4k4 = 0.053
v5k5 = 0.082361
v6k6 = 0.0032
v6Keq = 0.3975
v7k7 = 0.04
v8k8 = 3.6
cytoplasm = 1.0
mitochondrion = 1.0
end parameters

begin initial conditions
ATP[0] = ATPi
AcCoA[0] = AcCoAi
Cit[0] = Citi
KG[0] = KGi
NAD[0] = NADi
OAA[0] = OAAi
Pyr[0] = Pyri
DeltaPsi[0] = DeltaPsii
end initial conditions

begin initial values
ATPi = 3.536
AcCoAi = 0.063
Citi = 0.44
KGi = 0.225
NADi = 0.856
OAAi = 0.005
Pyri = 0.154
DeltaPsii = 150
end initial values

begin assignment rules
NADH := Nt - NAD[t]
ADP := At - ATP[t]
DeltaGtransport := 1.2*F*DeltaPsi[t]
ATPcrit := At/(1 + 1/(E^((3*DeltaGtransport)/(R*T))*iP*Kapp))
JATP := (-1 + 2/(1 + E^(b*(-ATPcrit + ATP[t]))))*kATP
Jresp := (kresp*(Nt - NAD[t]))/((1 + E^(a*(-DeltaPsim + DeltaPsi[t])))*(K + Nt - NAD[t]))
Jleak := kleak*DeltaPsi[t]
JANT := kANT*ATP[t]
end assignment rules

begin function definitions
end function definitions

begin events
end events

begin process annotations
end process annotations

begin species annotations
end species annotations

begin units
end units