begin name mueller1 end name begin reactions reaction1 {1.0}$EXTERNAL = {1.0}S10 reaction10 {1.0}S2 = {1.0}S14 reaction11 {1.0}S1 = {1.0}$EXTERNAL reaction12 {1.0}S2 = {1.0}S14 reaction13 {1.0}$EXTERNAL = {1.0}S14 reaction14 {1.0}S2 = {1.0}S1 reaction15 {1.0}S2 = {1.0}S1 + {1.0}S14 reaction16 {1.0}S1 = {1.0}S15 reaction17 {1.0}S2 = {1.0}S16 reaction18 {1.0}S5 = {1.0}S17 reaction19 {1.0}S10 = {1.0}$EXTERNAL reaction2 {1.0}$EXTERNAL = {1.0}S4 reaction20 {1.0}S13 = {1.0}$EXTERNAL reaction21 {1.0}S13 = {1.0}S18 reaction22 {1.0}S10 + {1.0}S12 = {1.0}S19 reaction23 {1.0}S4 + {1.0}S12 = {1.0}S20 reaction24 {1.0}S11 + {1.0}S13 = {1.0}S3 reaction25 {1.0}S12 = {1.0}S11 reaction26 {1.0}S12 = {1.0}$EXTERNAL reaction27 {1.0}S11 = {1.0}$EXTERNAL reaction28 {1.0}S12 = {1.0}$EXTERNAL reaction29 {1.0}S15 = {1.0}$EXTERNAL reaction3 {1.0}S3 = {1.0}S11 reaction30 {1.0}S16 = {1.0}S14 reaction31 {1.0}S15 = {1.0}$EXTERNAL reaction32 {1.0}S16 = {1.0}S14 reaction33 {1.0}S14 = {1.0}$EXTERNAL reaction34 {1.0}S16 = {1.0}S15 reaction35 {1.0}S1 + {1.0}S14 = {1.0}S2 reaction36 {1.0}S14 + {1.0}S15 = {1.0}S16 reaction37 {1.0}S16 = {1.0}S14 + {1.0}S15 reaction38 {1.0}S15 = {1.0}S21 reaction39 {1.0}S16 = {1.0}S14 + {1.0}S21 reaction4 {1.0}S4 = {1.0}$EXTERNAL reaction40 {1.0}S15 = {1.0}S1 reaction41 {1.0}S16 = {1.0}S2 reaction42 {1.0}S17 = {1.0}S22 reaction43 {1.0}S19 = {1.0}S12 reaction44 {1.0}S18 = {1.0}$EXTERNAL reaction45 {1.0}S20 = {1.0}S12 reaction46 {1.0}S18 = {1.0}$EXTERNAL reaction47 {1.0}S18 = {1.0}S13 reaction48 {1.0}S19 = {1.0}S10 + {1.0}S12 reaction49 {1.0}S11 + {1.0}S18 = {1.0}S23 reaction5 {1.0}$EXTERNAL = {1.0}S12 reaction50 {1.0}S20 = {1.0}S4 + {1.0}S12 reaction51 {1.0}S19 = {1.0}S24 reaction52 {1.0}S20 = {1.0}S3 reaction53 {1.0}S19 = {1.0}$EXTERNAL reaction54 {1.0}S20 = {1.0}$EXTERNAL reaction55 {1.0}S21 = {1.0}$EXTERNAL reaction56 {1.0}S21 = {1.0}$EXTERNAL reaction57 {1.0}S21 = {1.0}S15 reaction58 {1.0}S22 = {1.0}S25 reaction59 {1.0}S24 = {1.0}$EXTERNAL reaction6 {1.0}S3 = {1.0}S11 + {1.0}S13 reaction60 {1.0}S23 = {1.0}S11 reaction61 {1.0}S24 = {1.0}S11 + {1.0}S26 reaction62 {1.0}S23 = {1.0}S11 + {1.0}S18 reaction63 {1.0}S23 = {1.0}S18 reaction64 {1.0}S24 = {1.0}S26 reaction65 {1.0}S25 = {1.0}S27 reaction66 {1.0}S26 = {1.0}$EXTERNAL reaction67 {1.0}S11 + {1.0}S26 = {1.0}S24 reaction68 {1.0}S27 = {1.0}S28 reaction69 {1.0}S28 = {1.0}$EXTERNAL reaction7 {1.0}S3 = {1.0}S13 reaction8 {1.0}$EXTERNAL = {1.0}S1 reaction9 {1.0}S1 = {1.0}$EXTERNAL end reactions begin rate equations reaction1 = cell*Functionforreaction10[cell, ksc4, tf] reaction10 = Nucleus*Functionforreaction100[S2[t], cell, kdegrbbound] reaction11 = Nucleus*Functionforreaction110[S1[t], S11[t], cell, kdegrbp21] reaction12 = Nucleus*Functionforreaction120[S11[t], S2[t], cell, kdegrbp21] reaction13 = Nucleus*Functionforreaction130[S14[t], cell, kse2fe2f, kse2fmyc, tf] reaction14 = Nucleus*Functionforreaction140[S2[t], cell, kdege2fbound] reaction15 = Nucleus*Functionforreaction150[S2[t], cell, kdrbe2f] reaction16 = Nucleus*Functionforreaction160[Kmprb, S1[t], S24[t], cell, kcatprbc4, nrb] reaction17 = Nucleus*Functionforreaction170[Kmprb, S2[t], S24[t], cell, kcatprbc4, nrb] reaction18 = Nucleus*Functionforreaction180[S14[t], S18[t], S5[t], cell, kdna] reaction19 = cell*Functionforreaction190[S10[t], cell, gsk3b, kdegc4, kdegc4gsk3b] reaction2 = cell*Functionforreaction20[S14[t], S16[t], cell, ksc2e2f, ksc2myc, tf] reaction20 = Nucleus*Functionforreaction200[S13[t], cell, gsk3b, kdegc2, kdegc2gsk3b] reaction21 = Nucleus*Functionforreaction210[S13[t], cell, kpc2cak] reaction22 = cell*Functionforreaction220[S10[t], S12[t], cell, kbp21c4] reaction23 = cell*Functionforreaction230[S12[t], S4[t], cell, kbp21c2] reaction24 = Nucleus*Functionforreaction240[S11[t], S13[t], cell, kbp21c2] reaction25 = Functionforreaction250[S12[t], Vratio, cell, kimport] reaction26 = cell*Functionforreaction260[S12[t], Vratio, cell, kimport] reaction27 = Nucleus*Functionforreaction270[S11[t], cell, erk, gsk3b, kdegp21erk, kdegp21gsk3b] reaction28 = cell*Functionforreaction280[S12[t], cell, erk, gsk3b, kdegp21erk, kdegp21gsk3b] reaction29 = Nucleus*Functionforreaction290[S15[t], cell, kdegrbfree] reaction3 = Nucleus*Functionforreaction30[S3[t], cell, gsk3b, kdegc2, kdegc2gsk3b] reaction30 = Nucleus*Functionforreaction300[S16[t], cell, kdegrbbound] reaction31 = Nucleus*Functionforreaction310[S11[t], S15[t], cell, kdegrbp21] reaction32 = Nucleus*Functionforreaction320[S11[t], S16[t], cell, kdegrbp21] reaction33 = Nucleus*Functionforreaction330[S14[t], cell, kdege2ffree] reaction34 = Nucleus*Functionforreaction340[S16[t], cell, kdege2fbound] reaction35 = Nucleus*Functionforreaction350[S1[t], S14[t], cell, kbrbe2f] reaction36 = Nucleus*Functionforreaction360[S14[t], S15[t], cell, kbrbpe2f] reaction37 = Nucleus*Functionforreaction370[S16[t], cell, kdrbpe2f] reaction38 = Nucleus*Functionforreaction380[Kmprb, S15[t], S18[t], cell, kcatprbc2, nrb] reaction39 = Nucleus*Functionforreaction390[Kmprb, S16[t], S18[t], cell, kcatprbc2, nrb] reaction4 = cell*Functionforreaction40[S4[t], cell, gsk3b, kdegc2, kdegc2gsk3b] reaction40 = Nucleus*Functionforreaction400[Kmdprb, S15[t], S18[t], cell, kcatdprbc4, kinhpp1, nrb] reaction41 = Nucleus*Functionforreaction410[Kmdprb, S16[t], S18[t], cell, kcatdprbc4, kinhpp1, nrb] reaction42 = Nucleus*Functionforreaction420[S17[t], cell, kdelay] reaction43 = cell*Functionforreaction430[S19[t], cell, gsk3b, kdegc4, kdegc4gsk3b] reaction44 = Nucleus*Functionforreaction440[S18[t], cell, gsk3b, kdegc2, kdegc2gsk3b] reaction45 = cell*Functionforreaction450[S20[t], cell, gsk3b, kdegc2, kdegc2gsk3b] reaction46 = Nucleus*Functionforreaction460[S18[t], cell, gsk3b, kdegc2c2gsk3b] reaction47 = Nucleus*Functionforreaction470[S18[t], cell, kdpc2cak] reaction48 = cell*Functionforreaction480[S19[t], cell, kdp21c4] reaction49 = Nucleus*Functionforreaction490[S11[t], S18[t], cell, kbp21c2] reaction5 = cell*Functionforreaction50[S14[t], cell, ksp21e2f, ksp21p53, tfp21] reaction50 = cell*Functionforreaction500[S20[t], cell, kdp21c2] reaction51 = Functionforreaction510[S19[t], Vratio, cell, kimport] reaction52 = Functionforreaction520[S20[t], Vratio, cell, kimport] reaction53 = cell*Functionforreaction530[S19[t], Vratio, cell, kimport] reaction54 = cell*Functionforreaction540[S20[t], Vratio, cell, kimport] reaction55 = Nucleus*Functionforreaction550[S21[t], cell, kdegrbfree] reaction56 = Nucleus*Functionforreaction560[S11[t], S21[t], cell, kdegrbp21] reaction57 = Nucleus*Functionforreaction570[Kmdprb, S18[t], S21[t], cell, kcatdprbc2, kinhpp1, nrb] reaction58 = Nucleus*Functionforreaction580[S22[t], cell, kdelay] reaction59 = Nucleus*Functionforreaction590[S24[t], cell, kdegc4] reaction6 = Nucleus*Functionforreaction60[S3[t], cell, kdp21c2] reaction60 = Nucleus*Functionforreaction600[S23[t], cell, gsk3b, kdegc2, kdegc2gsk3b] reaction61 = Nucleus*Functionforreaction610[S24[t], cell, kdp21c4] reaction62 = Nucleus*Functionforreaction620[S23[t], cell, kdp21c2] reaction63 = Nucleus*Functionforreaction630[S14[t], S18[t], S23[t], cell, erk, kdegp21c2skp2, kdegp21erkskp2, kdegp21skp2] reaction64 = Nucleus*Functionforreaction640[S14[t], S18[t], S24[t], cell, erk, kdegp21c2skp2, kdegp21erkskp2, kdegp21skp2] reaction65 = Nucleus*Functionforreaction650[S25[t], cell, kdelay] reaction66 = Nucleus*Functionforreaction660[S26[t], cell, gsk3b, kdegc4, kdegc4gsk3b] reaction67 = Nucleus*Functionforreaction670[S11[t], S26[t], cell, kbp21c4] reaction68 = Nucleus*Functionforreaction680[S27[t], cell, kdelay] reaction69 = Nucleus*Functionforreaction690[S28[t], cell, kdelay] reaction7 = Nucleus*Functionforreaction70[S14[t], S18[t], S3[t], cell, erk, kdegp21c2skp2, kdegp21erkskp2, kdegp21skp2] reaction8 = Nucleus*Functionforreaction80[S14[t], cell, ksrb, ksrbe2f] reaction9 = Nucleus*Functionforreaction90[S1[t], cell, kdegrbfree] end rate equations begin parameters Kdp21c2 = 0.0100024233821379 Kdp21c4 = 99.9965429570432 Kdrbe2f = 50.002528361226 Kdrbpe2f = 481.484530572552 Kmdprb = 0.118988383643671 Kmprb = 2.03458881189349 Vcyto = 12.67 Vnuc = 0.25 aakt = 0.53 aerk = 0.16 atf = 0.601288203846636 kdelay = 23.6658781343201 kdna = 0.00949790539669408 kbp21c2 = 997.938141166465 kbp21c4 = 14.3083360067931 kbrbe2f = 229.976400323907 kbrbpe2f = 182.218452288549 kc2cak = 0.316993285134466 kcatdprbc2 = 0.00313841707547858 kcatdprbc4 = 2892.0219338341 kcatprbc2 = 7142308.07232621 kcatprbc4 = 2797.82326282727 kdegc2 = 0.225746618767114 kdegc2c2gsk3b = 5.58835572681068 kdegc2gsk3b = "1.55090179808215e-05" kdegc4 = 1.01433121526038 kdegc4gsk3b = 0.107637073030656 kdege2fbound = 0.0999954023364359 kdegp21c2skp2 = 0.040108041739907 kdegp21erk = 0.736488746268804 kdegp21erkskp2 = 0.000282976267377082 kdegp21gsk3b = 0.00464010657330714 kdegp21skp2 = 0.750574831653576 kdegrbbound = 0.0889964132806627 kdegrbp21 = 0.863570809432207 kdege2fplus = "4.18153340918872e-05" kdegrbplus = 0.257763482477731 kdpc2cak = 101.282119534273 ki = 0.0918326575000322 kinhp21akt = 0.439662894183616 kinhpp1 = 16634.9400020267 ksc2e2f = 2.19944932286058 ksc2myc = 0.157511710670132 ksc4 = 14298.6715905912 kse2fe2f = 0.459601740303536 kse2fmyc = "2.49174531457788e-06" ksp21e2f = 0.811617200647839 ksp21p53 = "3.84136205729286e-06" ksrb = 72.5245257602228 ksrbe2f = 20.0129834334888 nakt = 1.0957 nerk = 1.14663416967514 nrb = 3.0 pakt = 0.0348 perk = 0.00857314138104046 scalePhosRbS788 = 0.673907327042472 scalePhosRbS800 = 0.82377467648995 scaleTotCDK2T160 = 2.728395741944 scaleTotE2F = 28.7418 scaleTotRb = 0.2605 scaleTotcycDCDK4 = 0.5651 scaleTotcycECDK2 = 0.1889 scaleTotp21 = 0.1728 scaleTotp21CDK2 = 0.339790715037712 Nucleus = 1.0 cell = 1.0 end parameters begin initial conditions S1[0] = S1i S10[0] = S10i S11[0] = S11i S12[0] = S12i S13[0] = S13i S14[0] = S14i S15[0] = S15i S16[0] = S16i S17[0] = S17i S18[0] = S18i S19[0] = S19i S2[0] = S2i S20[0] = S20i S21[0] = S21i S22[0] = S22i S23[0] = S23i S24[0] = S24i S25[0] = S25i S26[0] = S26i S27[0] = S27i S28[0] = S28i S3[0] = S3i S4[0] = S4i S5[0] = S5i end initial conditions begin initial values S1i = 25.5914 S10i = 0.0 S11i = 0.0 S12i = 0.0 S13i = 0.0 S14i = 0.0 S15i = 0.0 S16i = 0.0 S17i = 0.0 S18i = 0.0 S19i = 0.0 S2i = 0.0601 S20i = 0.0 S21i = 0.0 S22i = 0.0 S23i = 0.0 S24i = 0.0 S25i = 0.0 S26i = 0.0 S27i = 0.0 S28i = 0.0 S3i = 6.2223 S4i = 0.415 S5i = 1.0 end initial values begin assignment rules kdege2ffree := kdege2fplus + kdege2fbound kcatprbc4 := (1 - inhc4d1)*kcatprbc4 kdrbpe2f := kbrbpe2f*Kdrbpe2f kdrbe2f := kbrbe2f*Kdrbe2f hgf := Piecewise[{{0, t < -1}}, Piecewise[{{0, t < 24}}, 1]] inhp53 := 0 inherk := 0 inhakt := 0 inhc4d1 := 0 ObsTotE2Fobs := ((scaleTotE2F + scaleTotRb)*Vnuc*(S14[t] + S16[t] + S2[t]))/(Vcyto + Vnuc) ObsTotCDK2T160obs := (scaleTotCDK2T160*Vnuc*(S18[t] + S23[t]))/(Vcyto + Vnuc) ObsTotCycDCDK4obs := (scaleTotcycDCDK4*(Vcyto*S19[t] + Vnuc*S24[t]))/(Vcyto + Vnuc) ObsPhosRbS788obs := (scalePhosRbS788*Vnuc*(S15[t] + S16[t] + S21[t]))/(Vcyto + Vnuc) ObsPhosRbS800obs := (scalePhosRbS800*Vnuc*S21[t])/(Vcyto + Vnuc) ObsDNAContentobs := 2 - S17[t] - S22[t] - S25[t] - S27[t] - S28[t] - S5[t] Vratio := Vnuc/Vcyto gsk3b := 1 - akt tf := atf + (1 - atf)*erk*(1 - gsk3b) kpc2cak := kc2cak + kdpc2cak kdp21c4 := kbp21c4*Kdp21c4 kdp21c2 := kbp21c2*Kdp21c2 kdegrbfree := kdegrbplus + kdegrbbound kimport := ki/(1 + akt*kinhp21akt) tfp21 := (1 - inhp53)*tf akt := (1 - inhakt)*(aakt + ((1 - aakt)*hgf^nakt*(1 + pakt^nakt))/(hgf^nakt + pakt^nakt)) erk := (1 - inherk)*(aerk + ((1 - aerk)*hgf^nerk*(1 + perk^nerk))/(hgf^nerk + perk^nerk)) ObsTotRbobs := (scaleTotRb*Vnuc*(S1[t] + S15[t] + S16[t] + S2[t] + S21[t]))/(Vcyto + Vnuc) ObsTotP21obs := (scaleTotp21*(Vcyto*(S12[t] + S19[t] + S20[t]) + Vnuc*(S11[t] + S23[t] + S24[t] + S3[t])))/(Vcyto + Vnuc) ObsTotCycECDK2obs := (scaleTotcycECDK2*(Vnuc*(S13[t] + S18[t] + S23[t] + S3[t]) + Vcyto*(S20[t] + S4[t])))/(Vcyto + Vnuc) ObsCDK2P21obs := (scaleTotp21CDK2*(Vcyto*S20[t] + Vnuc*(S23[t] + S3[t])))/(Vcyto + Vnuc) end assignment rules begin function definitions Functionforreaction230[S12_,S4_,cell_,kbp21c2_] := (kbp21c2*S12*S4)/cell Functionforreaction10[cell_,ksc4_,tf_] := (ksc4*tf)/cell Functionforreaction20[S14_,S16_,cell_,ksc2e2f_,ksc2myc_,tf_] := (ksc2e2f*(S14 + S16) + ksc2myc*tf)/cell Functionforreaction580[S22_,cell_,kdelay_] := (kdelay*S22)/cell Functionforreaction590[S24_,cell_,kdegc4_] := (kdegc4*S24)/cell Functionforreaction30[S3_,cell_,gsk3b_,kdegc2_,kdegc2gsk3b_] := ((kdegc2 + gsk3b*kdegc2gsk3b)*S3)/cell Functionforreaction40[S4_,cell_,gsk3b_,kdegc2_,kdegc2gsk3b_] := ((kdegc2 + gsk3b*kdegc2gsk3b)*S4)/cell Functionforreaction50[S14_,cell_,ksp21e2f_,ksp21p53_,tfp21_] := ((ksp21p53 + ksp21e2f*S14)*tfp21)/cell Functionforreaction60[S3_,cell_,kdp21c2_] := (kdp21c2*S3)/cell Functionforreaction90[S1_,cell_,kdegrbfree_] := (kdegrbfree*S1)/cell Functionforreaction100[S2_,cell_,kdegrbbound_] := (kdegrbbound*S2)/cell Functionforreaction110[S1_,S11_,cell_,kdegrbp21_] := (kdegrbp21*S1*S11)/cell Functionforreaction120[S11_,S2_,cell_,kdegrbp21_] := (kdegrbp21*S11*S2)/cell Functionforreaction130[S14_,cell_,kse2fe2f_,kse2fmyc_,tf_] := ((kse2fmyc + kse2fe2f*S14)*tf)/cell Functionforreaction140[S2_,cell_,kdege2fbound_] := (kdege2fbound*S2)/cell Functionforreaction150[S2_,cell_,kdrbe2f_] := (kdrbe2f*S2)/cell Functionforreaction170[Kmprb_,S2_,S24_,cell_,kcatprbc4_,nrb_] := (kcatprbc4*S2^nrb*S24)/(cell*(Kmprb^nrb + S2^nrb)) Functionforreaction180[S14_,S18_,S5_,cell_,kdna_] := (kdna*S14*S18*S5)/cell Functionforreaction190[S10_,cell_,gsk3b_,kdegc4_,kdegc4gsk3b_] := ((kdegc4 + gsk3b*kdegc4gsk3b)*S10)/cell Functionforreaction210[S13_,cell_,kpc2cak_] := (kpc2cak*S13)/cell Functionforreaction220[S10_,S12_,cell_,kbp21c4_] := (kbp21c4*S10*S12)/cell Functionforreaction240[S11_,S13_,cell_,kbp21c2_] := (kbp21c2*S11*S13)/cell Functionforreaction250[S12_,Vratio_,cell_,kimport_] := (kimport*S12)/(cell*Vratio) Functionforreaction260[S12_,Vratio_,cell_,kimport_] := (kimport*S12*(1 - Vratio^(-1)))/cell Functionforreaction280[S12_,cell_,erk_,gsk3b_,kdegp21erk_,kdegp21gsk3b_] := ((erk*kdegp21erk + gsk3b*kdegp21gsk3b)*S12)/cell Functionforreaction290[S15_,cell_,kdegrbfree_] := (kdegrbfree*S15)/cell Functionforreaction310[S11_,S15_,cell_,kdegrbp21_] := (kdegrbp21*S11*S15)/cell Functionforreaction320[S11_,S16_,cell_,kdegrbp21_] := (kdegrbp21*S11*S16)/cell Functionforreaction330[S14_,cell_,kdege2ffree_] := (kdege2ffree*S14)/cell Functionforreaction340[S16_,cell_,kdege2fbound_] := (kdege2fbound*S16)/cell Functionforreaction350[S1_,S14_,cell_,kbrbe2f_] := (kbrbe2f*S1*S14)/cell Functionforreaction370[S16_,cell_,kdrbpe2f_] := (kdrbpe2f*S16)/cell Functionforreaction380[Kmprb_,S15_,S18_,cell_,kcatprbc2_,nrb_] := (kcatprbc2*S15^nrb*S18)/(cell*(Kmprb^nrb + S15^nrb)) Functionforreaction390[Kmprb_,S16_,S18_,cell_,kcatprbc2_,nrb_] := (kcatprbc2*S16^nrb*S18)/(cell*(Kmprb^nrb + S16^nrb)) Functionforreaction400[Kmdprb_,S15_,S18_,cell_,kcatdprbc4_,kinhpp1_,nrb_] := (kcatdprbc4*S15^nrb)/(cell*(Kmdprb^nrb + S15^nrb)*(1 + kinhpp1*S18)) Functionforreaction420[S17_,cell_,kdelay_] := (kdelay*S17)/cell Functionforreaction410[Kmdprb_,S16_,S18_,cell_,kcatdprbc4_,kinhpp1_,nrb_] := (kcatdprbc4*S16^nrb)/(cell*(Kmdprb^nrb + S16^nrb)*(1 + kinhpp1*S18)) Functionforreaction500[S20_,cell_,kdp21c2_] := (kdp21c2*S20)/cell Functionforreaction490[S11_,S18_,cell_,kbp21c2_] := (kbp21c2*S11*S18)/cell Functionforreaction480[S19_,cell_,kdp21c4_] := (kdp21c4*S19)/cell Functionforreaction470[S18_,cell_,kdpc2cak_] := (kdpc2cak*S18)/cell Functionforreaction460[S18_,cell_,gsk3b_,kdegc2c2gsk3b_] := (gsk3b*kdegc2c2gsk3b*S18)/cell Functionforreaction450[S20_,cell_,gsk3b_,kdegc2_,kdegc2gsk3b_] := ((kdegc2 + gsk3b*kdegc2gsk3b)*S20)/cell Functionforreaction440[S18_,cell_,gsk3b_,kdegc2_,kdegc2gsk3b_] := ((kdegc2 + gsk3b*kdegc2gsk3b)*S18)/cell Functionforreaction430[S19_,cell_,gsk3b_,kdegc4_,kdegc4gsk3b_] := ((kdegc4 + gsk3b*kdegc4gsk3b)*S19)/cell Functionforreaction620[S23_,cell_,kdp21c2_] := (kdp21c2*S23)/cell Functionforreaction610[S24_,cell_,kdp21c4_] := (kdp21c4*S24)/cell Functionforreaction600[S23_,cell_,gsk3b_,kdegc2_,kdegc2gsk3b_] := ((kdegc2 + gsk3b*kdegc2gsk3b)*S23)/cell Functionforreaction570[Kmdprb_,S18_,S21_,cell_,kcatdprbc2_,kinhpp1_,nrb_] := (kcatdprbc2*S21^nrb)/(cell*(1 + kinhpp1*S18)*(Kmdprb^nrb + S21^nrb)) Functionforreaction560[S11_,S21_,cell_,kdegrbp21_] := (kdegrbp21*S11*S21)/cell Functionforreaction550[S21_,cell_,kdegrbfree_] := (kdegrbfree*S21)/cell Functionforreaction540[S20_,Vratio_,cell_,kimport_] := (kimport*S20*(1 - Vratio^(-1)))/cell Functionforreaction690[S28_,cell_,kdelay_] := (kdelay*S28)/cell Functionforreaction680[S27_,cell_,kdelay_] := (kdelay*S27)/cell Functionforreaction670[S11_,S26_,cell_,kbp21c4_] := (kbp21c4*S11*S26)/cell Functionforreaction660[S26_,cell_,gsk3b_,kdegc4_,kdegc4gsk3b_] := ((kdegc4 + gsk3b*kdegc4gsk3b)*S26)/cell Functionforreaction650[S25_,cell_,kdelay_] := (kdelay*S25)/cell Functionforreaction640[S14_,S18_,S24_,cell_,erk_,kdegp21c2skp2_,kdegp21erkskp2_,kdegp21skp2_] := (S14*(erk*kdegp21erkskp2 + kdegp21skp2 + kdegp21c2skp2*S18)*S24)/cell Functionforreaction630[S14_,S18_,S23_,cell_,erk_,kdegp21c2skp2_,kdegp21erkskp2_,kdegp21skp2_] := (S14*(erk*kdegp21erkskp2 + kdegp21skp2 + kdegp21c2skp2*S18)*S23)/cell Functionforreaction530[S19_,Vratio_,cell_,kimport_] := (kimport*S19*(1 - Vratio^(-1)))/cell Functionforreaction520[S20_,Vratio_,cell_,kimport_] := (kimport*S20)/(cell*Vratio) Functionforreaction510[S19_,Vratio_,cell_,kimport_] := (kimport*S19)/(cell*Vratio) Functionforreaction360[S14_,S15_,cell_,kbrbpe2f_] := (kbrbpe2f*S14*S15)/cell Functionforreaction300[S16_,cell_,kdegrbbound_] := (kdegrbbound*S16)/cell Functionforreaction270[S11_,cell_,erk_,gsk3b_,kdegp21erk_,kdegp21gsk3b_] := ((erk*kdegp21erk + gsk3b*kdegp21gsk3b)*S11)/cell Functionforreaction200[S13_,cell_,gsk3b_,kdegc2_,kdegc2gsk3b_] := ((kdegc2 + gsk3b*kdegc2gsk3b)*S13)/cell Functionforreaction160[Kmprb_,S1_,S24_,cell_,kcatprbc4_,nrb_] := (kcatprbc4*S1^nrb*S24)/(cell*(Kmprb^nrb + S1^nrb)) Functionforreaction80[S14_,cell_,ksrb_,ksrbe2f_] := (ksrb + ksrbe2f*S14)/cell Functionforreaction70[S14_,S18_,S3_,cell_,erk_,kdegp21c2skp2_,kdegp21erkskp2_,kdegp21skp2_] := (S14*(erk*kdegp21erkskp2 + kdegp21skp2 + kdegp21c2skp2*S18)*S3)/cell end function definitions begin events end events begin process annotations end process annotations begin species annotations ObsDNAContent_obs http://identifiers.org/chebi/CHEBI:16991 ObsTotCycDCDK4_obs http://identifiers.org/go/GO:0097128 ObsTotCycECDK2_obs http://identifiers.org/go/GO:0097134 ObsTotE2F_obs http://identifiers.org/uniprot/Q61501 ObsTotP21_obs http://identifiers.org/uniprot/P46414 ObsTotRb_obs http://identifiers.org/uniprot/P13405 S10 http://identifiers.org/go/GO:0097128 S11 http://identifiers.org/uniprot/P46414 S12 http://identifiers.org/uniprot/P46414 S14 http://identifiers.org/uniprot/Q61501 S17 http://identifiers.org/go/GO:0036387 S22 http://identifiers.org/go/GO:0036387 S25 http://identifiers.org/go/GO:0036387 S26 http://identifiers.org/go/GO:0097128 S27 http://identifiers.org/go/GO:0036387 S28 http://identifiers.org/go/GO:0036387 S5 http://identifiers.org/go/GO:0036387 hgf http://identifiers.org/uniprot/Q08048 inhakt http://identifiers.org/uniprot/P31750 inhc4d1 http://identifiers.org/go/GO:0097128 inherk http://identifiers.org/uniprot/Q63844 inhp53 http://identifiers.org/uniprot/P02340 end species annotations begin units end units