begin name
levchenko2
end name

begin reactions
Reaction1 {1.0}RAF + {1.0}RAFK = {1.0}RAFRAFK
Reaction10 {1.0}MEKp + {1.0}MEKPH = {1.0}MEKpMEKPH
Reaction11 {1.0}MEKpMEKPH = {1.0}MEKp + {1.0}MEKPH
Reaction12 {1.0}MEKpMEKPH = {1.0}MEK + {1.0}MEKPH
Reaction13 {1.0}MEKp + {1.0}RAFp = {1.0}MEKpRAFp
Reaction14 {1.0}MEKpRAFp = {1.0}MEKp + {1.0}RAFp
Reaction15 {1.0}MEKpRAFp = {1.0}MEKpp + {1.0}RAFp
Reaction16 {1.0}MEKPH + {1.0}MEKpp = {1.0}MEKppMEKPH
Reaction17 {1.0}MEKppMEKPH = {1.0}MEKPH + {1.0}MEKpp
Reaction18 {1.0}MEKppMEKPH = {1.0}MEKp + {1.0}MEKPH
Reaction19 {1.0}MAPK + {1.0}MEKpp = {1.0}MAPKMEKpp
Reaction2 {1.0}RAFRAFK = {1.0}RAF + {1.0}RAFK
Reaction20 {1.0}MAPKMEKpp = {1.0}MAPK + {1.0}MEKpp
Reaction21 {1.0}MAPKMEKpp = {1.0}MAPKp + {1.0}MEKpp
Reaction22 {1.0}MAPKp + {1.0}MAPKPH = {1.0}MAPKpMAPKPH
Reaction23 {1.0}MAPKpMAPKPH = {1.0}MAPKp + {1.0}MAPKPH
Reaction24 {1.0}MAPKpMAPKPH = {1.0}MAPK + {1.0}MAPKPH
Reaction25 {1.0}MAPKp + {1.0}MEKpp = {1.0}MAPKpMEKpp
Reaction26 {1.0}MAPKpMEKpp = {1.0}MAPKp + {1.0}MEKpp
Reaction27 {1.0}MAPKpMEKpp = {1.0}MAPKpp + {1.0}MEKpp
Reaction28 {1.0}MAPKPH + {1.0}MAPKpp = {1.0}MAPKppMAPKPH
Reaction29 {1.0}MAPKppMAPKPH = {1.0}MAPKPH + {1.0}MAPKpp
Reaction3 {1.0}RAFRAFK = {1.0}RAFK + {1.0}RAFp
Reaction30 {1.0}MAPKppMAPKPH = {1.0}MAPKp + {1.0}MAPKPH
Reaction4 {1.0}RAFp + {1.0}RAFPH = {1.0}RAFpRAFPH
Reaction5 {1.0}RAFpRAFPH = {1.0}RAFp + {1.0}RAFPH
Reaction6 {1.0}RAFpRAFPH = {1.0}RAF + {1.0}RAFPH
Reaction7 {1.0}MEK + {1.0}RAFp = {1.0}MEKRAFp
Reaction8 {1.0}MEKRAFp = {1.0}MEK + {1.0}RAFp
Reaction9 {1.0}MEKRAFp = {1.0}MEKp + {1.0}RAFp
vENZC1NEG {1.0}C1 = {1.0}$EXTERNAL
vENZC1POS {1.0}$EXTERNAL = {1.0}C1
vENZC2NEG {1.0}C2 = {1.0}$EXTERNAL
vENZC2POS {1.0}$EXTERNAL = {1.0}C2
vENZC3NEG {1.0}C3 = {1.0}$EXTERNAL
vENZC3POS {1.0}$EXTERNAL = {1.0}C3
vENZC4NEG {1.0}C4 = {1.0}$EXTERNAL
vENZC4POS {1.0}$EXTERNAL = {1.0}C4
vENZC5NEG {1.0}C5 = {1.0}$EXTERNAL
vENZC5POS {1.0}$EXTERNAL = {1.0}C5
vENZC6NEG {1.0}C6 = {1.0}$EXTERNAL
vENZC6POS {1.0}$EXTERNAL = {1.0}C6
vENZC7NEG {1.0}C7 = {1.0}$EXTERNAL
vENZC7POS {1.0}$EXTERNAL = {1.0}C7
vENZC8NEG {1.0}C8 = {1.0}$EXTERNAL
vENZC8POS {1.0}$EXTERNAL = {1.0}C8
vENZC9NEG {1.0}C9 = {1.0}$EXTERNAL
vENZC9POS {1.0}$EXTERNAL = {1.0}C9
vENZSCAFMAPKPPPOS {1.0}$EXTERNAL = {1.0}MAPKpp
vENZSCAFMAPKNEG {1.0}MAPK = {1.0}$EXTERNAL
vENZSCAFMAPKPOS {1.0}$EXTERNAL = {1.0}MAPK
vENZSCAFMEKPPPOS {1.0}$EXTERNAL = {1.0}MEKpp
vENZSCAFMEKNEG {1.0}MEK = {1.0}$EXTERNAL
vENZSCAFMEKPOS {1.0}$EXTERNAL = {1.0}MEK
end reactions

begin rate equations
Reaction1 = Reaction1a1*RAF[t]*RAFK[t]
Reaction10 = Reaction10a4*MEKp[t]*MEKPH[t]
Reaction11 = Reaction11d4*MEKpMEKPH[t]
Reaction12 = Reaction12k4*MEKpMEKPH[t]
Reaction13 = Reaction13a5*MEKp[t]*RAFp[t]
Reaction14 = Reaction14d5*MEKpRAFp[t]
Reaction15 = Reaction15k5*MEKpRAFp[t]
Reaction16 = Reaction16a6*MEKPH[t]*MEKpp[t]
Reaction17 = Reaction17d6*MEKppMEKPH[t]
Reaction18 = Reaction18k6*MEKppMEKPH[t]
Reaction19 = Reaction19a7*MAPK[t]*MEKpp[t]
Reaction2 = Reaction2d1*RAFRAFK[t]
Reaction20 = Reaction20d7*MAPKMEKpp[t]
Reaction21 = Reaction21k7*MAPKMEKpp[t]
Reaction22 = Reaction22a8*MAPKp[t]*MAPKPH[t]
Reaction23 = Reaction23d8*MAPKpMAPKPH[t]
Reaction24 = Reaction24k8*MAPKpMAPKPH[t]
Reaction25 = Reaction25a9*MAPKp[t]*MEKpp[t]
Reaction26 = Reaction26d9*MAPKpMEKpp[t]
Reaction27 = Reaction27k9*MAPKpMEKpp[t]
Reaction28 = Reaction28a10*MAPKPH[t]*MAPKpp[t]
Reaction29 = Reaction29d10*MAPKppMAPKPH[t]
Reaction3 = Reaction3k1*RAFRAFK[t]
Reaction30 = Reaction30k10*MAPKppMAPKPH[t]
Reaction4 = Reaction4a2*RAFp[t]*RAFPH[t]
Reaction5 = Reaction5d2*RAFpRAFPH[t]
Reaction6 = Reaction6k2*RAFpRAFPH[t]
Reaction7 = Reaction7a3*MEK[t]*RAFp[t]
Reaction8 = Reaction8d3*MEKRAFp[t]
Reaction9 = Reaction9k3*MEKRAFp[t]
vENZC1NEG = C1[t]*(on2*MAPK[t] + on1*MEK[t])
vENZC1POS = of1*C2[t] + of3*C3[t] + of2*C4[t] + of4*C5[t]
vENZC2NEG = C2[t]*(of1 + on2*MAPK[t]) + kr1*C2[t]*RAFp[t]
vENZC2POS = of2*C6[t] + of4*C9[t] + on1*C1[t]*MEK[t]
vENZC3NEG = of3*C3[t] + on2*C3[t]*MAPK[t]
vENZC3POS = of2*C7[t] + of4*C8[t] + kr1*C2[t]*RAFp[t]
vENZC4NEG = C4[t]*(of2 + on1*MEK[t])
vENZC4POS = of1*C6[t] + of3*C7[t] + on2*C1[t]*MAPK[t]
vENZC5NEG = C5[t]*(of4 + on1*MEK[t])
vENZC5POS = of3*C8[t] + of1*C9[t]
vENZC6NEG = (of1 + of2)*C6[t] + kr1*C6[t]*RAFp[t]
vENZC6POS = on2*C2[t]*MAPK[t] + on1*C4[t]*MEK[t]
vENZC7NEG = kr2*C7[t] + of2*C7[t] + of3*C7[t]
vENZC7POS = on2*C3[t]*MAPK[t] + kr1*C6[t]*RAFp[t]
vENZC8NEG = (of3 + of4)*C8[t]
vENZC8POS = kr2*C7[t] + kr1*C9[t]*RAFp[t]
vENZC9NEG = (of1 + of4)*C9[t] + kr1*C9[t]*RAFp[t]
vENZC9POS = on1*C5[t]*MEK[t]
vENZSCAFMAPKPPPOS = of4*(C5[t] + C8[t] + C9[t])
vENZSCAFMAPKNEG = on2*(C1[t] + C2[t] + C3[t])*MAPK[t]
vENZSCAFMAPKPOS = of2*(C4[t] + C6[t] + C7[t])
vENZSCAFMEKPPPOS = of3*(C3[t] + C7[t] + C8[t])
vENZSCAFMEKNEG = on1*(C1[t] + C4[t] + C5[t])*MEK[t]
vENZSCAFMEKPOS = of1*(C2[t] + C6[t] + C9[t])
end rate equations

begin parameters
initialvalueC1Fraction = 1.0
initialvalueC2Fraction = 0.0
initialvalueC4Fraction = 0.0
initialvalueC6Fraction = 0.0
initialvalueMAPK = 0.4
initialvalueMEK = 0.2
initialvalueRAFK = 0.2
initialvalueTotalScaffold = 0.0
kr1 = 0.1
kr2 = 0.1
of1 = 0.05
of2 = 0.05
of3 = 0.05
of4 = 0.5
on1 = 10.0
on2 = 10.0
Reaction1a1 = 1.0
Reaction10a4 = 10.0
Reaction11d4 = 0.8
Reaction12k4 = 0.1
Reaction13a5 = 3.3
Reaction14d5 = 0.4
Reaction15k5 = 0.1
Reaction16a6 = 10.0
Reaction17d6 = 0.8
Reaction18k6 = 0.1
Reaction19a7 = 20.0
Reaction2d1 = 0.4
Reaction20d7 = 0.6
Reaction21k7 = 0.1
Reaction22a8 = 5.0
Reaction23d8 = 0.4
Reaction24k8 = 0.1
Reaction25a9 = 20.0
Reaction26d9 = 0.6
Reaction27k9 = 0.1
Reaction28a10 = 5.0
Reaction29d10 = 0.4
Reaction3k1 = 0.1
Reaction30k10 = 0.1
Reaction4a2 = 0.5
Reaction5d2 = 0.5
Reaction6k2 = 0.1
Reaction7a3 = 3.3
Reaction8d3 = 0.42
Reaction9k3 = 0.1
Cytoplasm = 1.0
end parameters

begin initial conditions
C1[0] = C1i
C2[0] = C2i
C3[0] = C3i
C4[0] = C4i
C5[0] = C5i
C6[0] = C6i
C7[0] = C7i
C8[0] = C8i
C9[0] = C9i
MAPK[0] = MAPKi
MAPKMEKpp[0] = MAPKMEKppi
MAPKPH[0] = MAPKPHi
MAPKp[0] = MAPKpi
MAPKpMAPKPH[0] = MAPKpMAPKPHi
MAPKpMEKpp[0] = MAPKpMEKppi
MAPKpp[0] = MAPKppi
MAPKppMAPKPH[0] = MAPKppMAPKPHi
MEK[0] = MEKi
MEKPH[0] = MEKPHi
MEKRAFp[0] = MEKRAFpi
MEKp[0] = MEKpi
MEKpMEKPH[0] = MEKpMEKPHi
MEKpRAFp[0] = MEKpRAFpi
MEKpp[0] = MEKppi
MEKppMEKPH[0] = MEKppMEKPHi
RAF[0] = RAFi
RAFK[0] = RAFKi
RAFPH[0] = RAFPHi
RAFRAFK[0] = RAFRAFKi
RAFp[0] = RAFpi
RAFpRAFPH[0] = RAFpRAFPHi
end initial conditions

begin initial values
C1i = 0
C2i = 0
C3i = 0.0
C4i = 0
C5i = 0.0
C6i = 0
C7i = 0.0
C8i = 0.0
C9i = 0.0
MAPKi = 0.4
MAPKMEKppi = 0.0
MAPKPHi = 0.3
MAPKpi = 0.0
MAPKpMAPKPHi = 0.0
MAPKpMEKppi = 0.0
MAPKppi = 0.0
MAPKppMAPKPHi = 0.0
MEKi = 0.2
MEKPHi = 0.2
MEKRAFpi = 0.0
MEKpi = 0.0
MEKpMEKPHi = 0.0
MEKpRAFpi = 0.0
MEKppi = 0.0
MEKppMEKPHi = 0.0
RAFi = 0.3
RAFKi = 0.2
RAFPHi = 0.3
RAFRAFKi = 0.0
RAFpi = 0.0
RAFpRAFPHi = 0.0
end initial values

begin assignment rules
end assignment rules

begin function definitions
end function definitions

begin events
end events

begin process annotations
end process annotations

begin species annotations
MAPKPH http://identifiers.org/uniprot/Q90W58
end species annotations

begin units
end units