#fisher1



#reactions
R1:
 {1.0}Act_C_Nuc + {1.0}NFAT_Pi_Nuc = {1.0}NFAT_Nuc + {1.0}Act_C_Nuc
  nucleus * (k1 * NFAT_Pi_Nuc - k2 * NFAT_Nuc)
R10:
 {1.0}NFAT_Pi_Cyt = {1.0}NFAT_Pi_Nuc
  cytosol * k3 * NFAT_Pi_Cyt - nucleus * k4 * NFAT_Pi_Nuc
R11:
 {1.0}NFAT_Act_C_Cyt = {1.0}NFAT_Cyt + {1.0}Act_C_Cyt
  cytosol * (k15 * NFAT_Act_C_Cyt - k16 * NFAT_Cyt * Act_C_Cyt)
R12:
 {1.0}Act_C_Cyt + {1.0}NFAT_Pi_Cyt = {1.0}NFAT_Cyt + {1.0}Act_C_Cyt
  cytosol * (k1 * NFAT_Pi_Cyt - k2 * NFAT_Cyt)
R13:
 {1.0}Inact_C_Cyt + {3.0}Ca_Cyt = {1.0}Act_C_Cyt
  cytosol * (k19 * Inact_C_Cyt * pow(Ca_Cyt, 3) - k20 * Act_C_Cyt)
R14:
 {1.0}Inact_C_Nuc + {3.0}Ca_Nuc = {1.0}Act_C_Nuc
  nucleus * (k19 * Inact_C_Nuc * pow(Ca_Nuc, 3) - k20 * Act_C_Nuc)
R15:
 {1.0}Inact_C_Cyt = {1.0}Inact_C_Nuc
  cytosol * k5 * Inact_C_Cyt - nucleus * k6 * Inact_C_Nuc
R16:
 {1.0}Ca_Cyt = {1.0}Ca_Nuc
  cytosol * k21 * Ca_Cyt - nucleus * k22 * Ca_Nuc
R17:
 {1.0}NFAT_Pi_Act_C_Cyt = {1.0}NFAT_Pi_Act_C_Nuc
  cytosol * k7 * NFAT_Pi_Act_C_Cyt - nucleus * k8 * NFAT_Pi_Act_C_Nuc
R2:
 {1.0}NFAT_Nuc + {1.0}Act_C_Nuc = {1.0}NFAT_Act_C_Nuc
  nucleus * (k16 * NFAT_Nuc * Act_C_Nuc - k15 * NFAT_Act_C_Nuc)
R3:
 {1.0}NFAT_Nuc = {1.0}NFAT_Cyt
  nucleus * k18 * NFAT_Nuc - cytosol * k17 * NFAT_Cyt
R4:
 {1.0}Act_C_Nuc = {1.0}Act_C_Cyt
  nucleus * k6 * Act_C_Nuc - cytosol * k5 * Act_C_Cyt
R5:
 {1.0}NFAT_Act_C_Nuc = {1.0}NFAT_Pi_Act_C_Nuc
  nucleus * (k14 * NFAT_Act_C_Nuc - k13 * NFAT_Pi_Act_C_Nuc)
R6:
 {1.0}NFAT_Pi_Act_C_Nuc = {1.0}NFAT_Pi_Nuc + {1.0}Act_C_Nuc
  nucleus * (k12 * NFAT_Pi_Act_C_Nuc - k11 * NFAT_Pi_Nuc * Act_C_Nuc)
R7:
 {1.0}NFAT_Act_C_Nuc = {1.0}NFAT_Act_C_Cyt
  nucleus * k10 * NFAT_Act_C_Nuc - cytosol * k9 * NFAT_Act_C_Cyt
R8:
 {1.0}NFAT_Act_C_Cyt = {1.0}NFAT_Pi_Act_C_Cyt
  cytosol * (k14 * NFAT_Act_C_Cyt - k13 * NFAT_Pi_Act_C_Cyt)
R9:
 {1.0}NFAT_Pi_Act_C_Cyt = {1.0}NFAT_Pi_Cyt + {1.0}Act_C_Cyt
  cytosol * (k12 * NFAT_Pi_Act_C_Cyt - k11 * NFAT_Pi_Cyt * Act_C_Cyt)


#parameters
k1 = 2.56e-05
k10 = 0.005
k11 = 6.63
k12 = 0.00168
k13 = 0.5
k14 = 0.00256
k15 = 0.00168
k16 = 6.63
k17 = 0.0015
k18 = 0.00096
k19 = 1.0
k2 = 0.00256
k20 = 1.0
k21 = 0.21
k22 = 0.5
k3 = 0.005
k4 = 0.5
k5 = 0.0019
k6 = 0.00092
k7 = 0.005
k8 = 0.5
k9 = 0.5
cytosol = 2.69e-13
nucleus = 1.13e-13


#initial values
Act_C_Cyt = 9.1e-06
Act_C_Nuc = 5.05e-05
Ca_Cyt = 1.0
Ca_Nuc = 1.0
Inact_C_Cyt = 0.0097108
Inact_C_Nuc = 0.049198
NFAT_Act_C_Cyt = 6.1e-06
NFAT_Act_C_Nuc = 0.0009477
NFAT_Cyt = 0.0001101
NFAT_Nuc = 0.0005219
NFAT_Pi_Act_C_Cyt = 2.2e-06
NFAT_Pi_Act_C_Nuc = 2.5e-06
NFAT_Pi_Cyt = 0.0094397
NFAT_Pi_Nuc = 0.0002272


#assignment rules