begin name
dupont2
end name

begin reactions
ProteinPhosphorylation {1.0}$EXTERNAL = {1.0}Wstar
R0 {1.0}$EXTERNAL = {1.0}Z
R1 {1.0}$EXTERNAL = {1.0}Z
R2 {1.0}Z = {1.0}Y
R3 {1.0}Y = {1.0}Z
Reff {1.0}Z = {1.0}$EXTERNAL
Rf {1.0}Y = {1.0}Z
end reactions

begin rate equations
ProteinPhosphorylation = (cytosol*vp*((vk*(1 - Wstar[t]))/(vp*(1 + K1 - Wstar[t])) - Wstar[t]/(K2 + Wstar[t])))/Wt[t]
R0 = cytosol*v0
R1 = cytosol*v1beta
R2 = (cytosol*Vm2*Z[t]^n)/(Kp^n + Z[t]^n)
R3 = (store*Vm3*Y[t]^m*Z[t]^p)/((Kr^m + Y[t]^m)*(KA^p + Z[t]^p))
Reff = cytosol*k*Z[t]
Rf = kf*store*Y[t]
end rate equations

begin parameters
K1 = 0.01
K2 = 0.01
KA = 0.9
Ka = 2.5
Kp = 1.0
Kr = 2.0
Vm2 = 65.0
Vm3 = 500.0
k = 10.0
kf = 1.0
m = 2.0
n = 2.0
p = 4.0
q = 1.0
v0 = 1.0
v1beta = 2.7
vMK = 20.0
vp = 2.5
cytosol = 1.0
store = 1.0
end parameters

begin initial conditions
Wstar[0] = Wstari
Wt[0] = Wti
Y[0] = Yi
Z[0] = Zi
end initial conditions

begin initial values
Wstari = 0.0
Wti = 1.0
Yi = 1.6
Zi = 0.15
end initial values

begin assignment rules
vk := (vMK*Z[t]^q)/(Ka^q + Z[t]^q)
end assignment rules

begin function definitions
end function definitions

begin events
end events

begin process annotations
Protein_Phosphorylation http://identifiers.org/go/GO:0042327
R0 http://identifiers.org/go/GO:0006816
R1 http://identifiers.org/go/GO:0005220
R2 http://identifiers.org/go/GO:0032470
R3 http://identifiers.org/go/GO:0048763
R3 http://identifiers.org/go/GO:0007204
R_eff http://identifiers.org/go/GO:0006816
Rf http://identifiers.org/go/GO:0006816
end process annotations

begin species annotations
Y http://identifiers.org/chebi/CHEBI:29108
Y http://identifiers.org/kegg.compound/C00076
Z http://identifiers.org/chebi/CHEBI:29108
Z http://identifiers.org/kegg.compound/C00076
end species annotations

begin units
end units