#calzone1



#reactions
Nuclei_1:
 $pool = {1.0}N
  0
R_1:
 $pool = {1.0}MPFc
  ksc * cytoplasm
R_10:
 {1.0}Stgm = $pool
  nuclei * (kdmp * Stgm / (Jm + Stgm) + kdm * Xp * Stgm)
R_12:
 $pool = {1.0}Stgc
  cytoplasm * ksstg * Stgm
R_13:
 {1.0}Stgc = {1.0}StgPc
  cytoplasm * ((kastgp + kastg * MPFc) * Stgc / (Jastg + Stgc))
R_14:
 {1.0}StgPc = {1.0}Stgc
  cytoplasm * (kistg * StgPc / (Jistg + StgPc))
R_15:
 {1.0}Stgc = $pool
  cytoplasm * kdstg * Stgc
R_16:
 {1.0}StgPc = $pool
  cytoplasm * kdstg * StgPc
R_19:
 {1.0}MPFc = $pool
  cytoplasm * (kt * MPFc * E_1 * N / (1 - N * E_1))
R_2:
 {1.0}preMPFc = {1.0}MPFc
  cytoplasm * (kstgp + kstg * StgPc) * preMPFc
R_20:
 {1.0}preMPFc = $pool
  cytoplasm * (kt * preMPFc * E_1 * N / (1 - N * E_1))
R_21:
 {1.0}Wee1Pn = $pool
  nuclei * koutw_1 * Wee1Pn
R_22:
 $pool = $pool
  cytoplasm * (kinw_1 * Wee1Pc * E_1 * N / (1 - N * E_1))
R_23:
 {1.0}Wee1n = $pool
  nuclei * koutw_1 * Wee1n
R_24:
 {1.0}Wee1c = $pool
  cytoplasm * (kinw_1 * Wee1c * E_1 * N / (1 - N * E_1))
R_25:
 {1.0}StgPn = $pool
  nuclei * kouts_1 * StgPn
R_26:
 {1.0}StgPc = $pool
  cytoplasm * (kins_1 * StgPc * E_1 * N / (1 - N * E_1))
R_27:
 {1.0}Stgn = $pool
  nuclei * kouts_1 * Stgn
R_28:
 {1.0}Stgc = $pool
  cytoplasm * (kins_1 * Stgc * E_1 * N / (1 - N * E_1))
R_29:
 {1.0}preMPFn = {1.0}MPFn
  nuclei * (kstgp + kstg * StgPn) * preMPFn
R_3:
 {1.0}MPFc = {1.0}preMPFc
  cytoplasm * (kweep + kwee * Wee1c) * MPFc
R_30:
 {1.0}MPFn = {1.0}preMPFn
  nuclei * (kweep + kwee * Wee1n) * MPFn
R_33:
 {1.0}preMPFn = $pool
  nuclei * (kdnp + kdn * FZYa) * preMPFn
R_34:
 {1.0}MPFn = $pool
  nuclei * (kdnp + kdn * FZYa) * MPFn
R_37:
 {1.0}IEa_1 = $pool
  nuclei * (kiie * IEa_1 / (Jiie + IEa_1))
R_38:
 $pool = {1.0}IEa_1
  nuclei * (kaie * (1 - IEa_1) * MPFn / (Jaie + 1 - IEa_1))
R_39:
 $pool = {1.0}FZYa
  nuclei * (kafzy * IEa_1 * (1 - FZYa) / (Jafzy + 1 - FZYa))
R_40:
 {1.0}FZYa = $pool
  nuclei * (kifzy * FZYa / (Jifzy + FZYa))
R_41:
 {1.0}Wee1n = {1.0}Wee1Pn
  nuclei * ((kiweep + kiwee * MPFn) * Wee1n / (Jiwee + Wee1n))
R_42:
 {1.0}Wee1Pn = {1.0}Wee1n
  nuclei * (kawee * Wee1Pn / (Jawee + Wee1Pn))
R_43:
 {1.0}Stgn = {1.0}StgPn
  nuclei * ((kastgp + kastg * MPFn) * Stgn / (Jastg + Stgn))
R_44:
 {1.0}StgPn = {1.0}Stgn
  nuclei * (kistg * StgPn / (Jistg + StgPn))
R_45:
 {1.0}Stgn = $pool
  nuclei * kdstg * Stgn
R_46:
 {1.0}StgPn = $pool
  nuclei * kdstg * StgPn
R_6:
 {1.0}preMPFc = $pool
  cytoplasm * kdc * preMPFc
R_7:
 {1.0}MPFc = $pool
  cytoplasm * kdc * MPFc
R_8:
 {1.0}Wee1c = $pool
  cytoplasm * ((kiweep + kiwee * MPFc) * Wee1c / (Jiwee + Wee1c))
R_9:
 $pool = {1.0}Wee1c
  cytoplasm * (kawee * Wee1Pc / (Jawee + Wee1Pc))
_16:
 $pool = {1.0}MPFn
  cytoplasm * kt * MPFc
_18:
 $pool = {1.0}preMPFn
  cytoplasm * kt * preMPFc
_182_1:
 $pool = $pool
  nuclei * (koutw_1 * Wee1Pn * N * E_1 / (1 - N * E_1))
_20:
 $pool = {1.0}Wee1Pn
  cytoplasm * kinw_1 * Wee1Pc
_22:
 $pool = {1.0}Wee1c
  nuclei * (koutw_1 * Wee1n * N * E_1 / (1 - N * E_1))
_24:
 $pool = {1.0}Wee1n
  cytoplasm * kinw_1 * Wee1c
_26:
 $pool = {1.0}StgPc
  nuclei * (kouts_1 * StgPn * E_1 * N / (1 - N * E_1))
_28:
 $pool = {1.0}StgPn
  cytoplasm * kins_1 * StgPc
_30:
 $pool = {1.0}Stgc
  nuclei * (kouts_1 * Stgn * E_1 * N / (1 - N * E_1))
_32:
 $pool = {1.0}Stgn
  cytoplasm * kins_1 * Stgc
_50:
 $pool = {1.0}Xm
  nuclei * ksxm_1 * N
_51:
 $pool = {1.0}Xp
  cytoplasm * ksxp_1 * Xm
exportofMPFfromnucleus_1:
 {1.0}MPFn = $pool
  nuclei * kout_1 * MPFn
exportofpreMPFnfromnucleus_1:
 {1.0}preMPFn = $pool
  nuclei * kout_1 * preMPFn
importofMPFintocytoplasm_1:
 $pool = {1.0}MPFc
  nuclei * (kout_1 * MPFn * E_1 * N / (1 - N * E_1))
importofpreMPFintocytoplaslm_1:
 $pool = {1.0}preMPFc
  nuclei * (kout_1 * preMPFn * N * E_1 / (1 - N * E_1))


#parameters
E_1 = 7e-05
Jafzy = 0.01
Jaie = 0.01
Jastg = 0.05
Jawee = 0.05
Jifzy = 0.01
Jiie = 0.01
Jistg = 0.05
Jiwee = 0.05
Jm = 0.05
StringT = 0.8
Wee1T = 0.8
factor_1 = 1.95
kafzy = 1.0
kaie = 1.0
kastg = 1.0
kastgp = 0.0
kawee = 0.3
kdc = 0.01
kdm = 0.2
kdmp = 0.002
kdn = 1.5
kdnp = 0.01
kdstg = 0.0
kez_1 = 0.5
kifzy = 0.2
kiie = 0.4
kins_1 = 0.08
kinw_1 = 0.04
kistg = 0.3
kiwee = 1.0
kiweep = 0.01
kout_1 = 0.0
kouts_1 = 0.02
koutw_1 = 0.01
ksc = 0.01
ksstg = 0.0
kstg = 2.0
kstgp = 0.2
ksxm_1 = 0.0005
ksxp_1 = 0.001
kt = 0.15
kwee = 1.0
kweep = 0.005
cytoplasm = 1.0
nuclei = 1.0


#initial values
FZYa = 0.0
IEa_1 = 0.0
MPFc = 1.0
MPFn = 0.0
N = 1.0
StgPc = 0.8
StgPn = 0.0
Stgc = 0.0
Stgm = 1.0
Stgn = 0.0
Wee1Pn = 0.0
Wee1c = 0.0
Wee1n = 0.0
Xm = 0.0
Xp = 0.0
preMPFc = 0.0
preMPFn = 0.0


#assignment rules
!F Wee1Pc = (Wee1T - N * E_1 * (Wee1n + Wee1Pn)) / (1.0 - N * E_1) - Wee1c
!F StgPT = (1.0 - N * E_1) * StgPc + N * E_1 * StgPn
!F CycBT = (1.0 - N * E_1) * (MPFc + preMPFc) + N * E_1 * (MPFn + preMPFn)