begin name
borghans2
end name

begin reactions
v2 {1.0}Z = {1.0}Y
v3 {1.0}Y = {1.0}Z
v4 {1.0}Y = {1.0}Z
v5 {1.0}Z = {1.0}EC
v6 {1.0}$EXTERNAL = {1.0}A
v7 {1.0}A = {1.0}$EXTERNAL
v8 {1.0}A = {1.0}$EXTERNAL
vin {1.0}EC = {1.0}Z
end reactions

begin rate equations
v2 = (intravesicular*v2Vm2*Z[t]^2)/(v2K2^2 + Z[t]^2)
v3 = (cytosol*v3Vm3*A[t]^4*Y[t]^2*Z[t]^4)/((v3Ka^4 + A[t]^4)*(v3Ky^2 + Y[t]^2)*(v3Kz^4 + Z[t]^4))
v4 = cytosol*v4Kf*Y[t]
v5 = extracellular*v5K*Z[t]
v6 = beta*cytosol*v6Vp
v7 = (cytosol*v7Vd*A[t]^2*Z[t]^v7n)/((v7Kp^2 + A[t]^2)*(v7Kd^v7n + Z[t]^v7n))
v8 = cytosol*v8epsilon*A[t]
vin = cytosol*(vinv0 + beta*vinv1)
end rate equations

begin parameters
beta = 0.5
vinv0 = 2.0
vinv1 = 1.0
v2Vm2 = 6.5
v2K2 = 0.1
v3Vm3 = 19.5
v3Ka = 0.2
v3Ky = 0.2
v3Kz = 0.3
v4Kf = 1.0
v5K = 10.0
v6Vp = 2.5
v7Vd = 80.0
v7Kp = 1.0
v7Kd = 0.4
v7n = 4.0
v8epsilon = 0.1
cytosol = 1.0
extracellular = 1.0
intravesicular = 1.0
end parameters

begin initial conditions
A[0] = Ai
EC[0] = ECi
Y[0] = Yi
Z[0] = Zi
end initial conditions

begin initial values
Ai = 0.45
ECi = 1000.0
Yi = 0.36
Zi = 0.0
end initial values

begin assignment rules
end assignment rules

begin function definitions
end function definitions

begin events
end events

begin process annotations
end process annotations

begin species annotations
A http://identifiers.org/chebi/CHEBI:16595
A http://identifiers.org/kegg.compound/C01245
EC http://identifiers.org/chebi/CHEBI:29108
EC http://identifiers.org/kegg.compound/C00076
Y http://identifiers.org/chebi/CHEBI:29108
Y http://identifiers.org/kegg.compound/C00076
Z http://identifiers.org/chebi/CHEBI:29108
Z http://identifiers.org/kegg.compound/C00076
end species annotations

begin units
end units