begin name arnold8 end name begin reactions ATPS {1.0}ADP + {1.0}Pi = {1.0}ATP PGAred {1.0}PGA + {1.0}ATP = {1.0}ADP + {1.0}TP + {1.0}Pi RuBPreg {1.0}Ru5P + {1.0}ATP = {1.0}RuBP + {1.0}ADP RuBisCO {1.0}RuBP = {2.0}PGA TPT {1.0}TP + {1.0}$Pic = {1.0}$TPc + {1.0}Pi TPred {5.0}TP = {3.0}Ru5P + {2.0}Pi end reactions begin rate equations ATPS = chloroplast*function10[V6, ADP[t], Pi[t], ATPSK1, ATPSK2] PGAred = chloroplast*function6[PGAredVm, PGA[t], ATP[t], ADP[t], TP[t], Pi[t], PGAredk, PGAredK1, PGAredK2] RuBPreg = chloroplast*function8[RuBPregVm, Ru5P[t], ATP[t], RuBPregK1, RuBPregK2, RuBPregK3, Pi[t]] RuBisCO = chloroplast*function5[RuBisCOk, ERuBisCO, totRuBP, RuBisCOK] TPT = function9[TP[t], Pic, TPc, Pi[t], TPTK2, TPTK1, TPTVm] TPred = chloroplast*function7[TPredVm, TP[t], TPredK] end rate equations begin parameters P0 = 16.0 V6 = 5.8801285588795 ERuBisCO = 3.56 Pic = 1.4 TPc = 0.2 RuBisCOk = 0.504 RuBisCOK = 0.04 PGAredVm = 3.49 PGAredk = 14.0 PGAredK1 = 1.0 PGAredK2 = 1.0 TPredVm = 1.06 TPredK = 0.4 RuBPregVm = 4.81 RuBPregK1 = 0.05 RuBPregK2 = 0.5 RuBPregK3 = 0.05 TPTK2 = 0.25 TPTK1 = 0.08 TPTVm = 3.3 ATPSK1 = 0.08 ATPSK2 = 0.5 chloroplast = 1.0 cytosol = 1.0 end parameters begin initial conditions ADP[0] = ADPi ATP[0] = ATPi PGA[0] = PGAi Pi[0] = Pii Ru5P[0] = Ru5Pi RuBP[0] = RuBPi TP[0] = TPi end initial conditions begin initial values ADPi = 0.82 ATPi = 0.68 PGAi = 2.4 Pii = 5.0 Ru5Pi = 0.0500747384155456 RuBPi = 2.0 TPi = 0.5 end initial values begin assignment rules totRuBP := (P0 - ATP[t] - PGA[t] - Pi[t] - Ru5P[t] - TP[t])/2 end assignment rules begin function definitions function9[S1_,S2_,P1_,P2_,K2_,K1_,Vm_] := ((-(P1*P2) + S1*S2)*Vm)/(K2*(P1 + S1) + K1*(P2 + S2) + K1*K2*(P2/K2 + S1/K1)*(P1/K1 + S2/K2)) function10[Vm_,s1_,s2_,K1_,K2_] := (s1*s2*Vm)/((K1 + s1)*(K2 + s2)) function8[Vm_,S1_,S2_,K1_,K2_,K3_,R_] := (S1*S2*Vm)/(K1*K2 + K3*R + K2*S2 + S1*S2) function7[Vm_,S_,K_] := (S*Vm)/(K + S) function5[k_,E_,S_,K_] := (k*(E + K + S - Sqrt[-4*E*S + (E + K + S)^2]))/2 function6[Vm_,S1_,S2_,P1_,P2_,P3_,k_,K1_,K2_] := ((-((P1*P2*P3)/k) + S1*S2)*Vm)/(K1 + (P1*P2*P3)/k + (K1*S1*S2)/K2) end function definitions begin events end events begin process annotations end process annotations begin species annotations ADP http://identifiers.org/chebi/CHEBI:16761 end species annotations begin units end units