#akman2



#reactions
FCdeg:
 {1.0}FC = $pool
  vd * FC
FCpdeg:
 {1.0}FCp = $pool
  vdp * FCp
FCptrl:
 $pool = {1.0}FCp
  ksp * MF
FCptrs:
 {1.0}FCp = {1.0}FNp
  k1np * FCp - k2np * FNp
FCtrl:
 $pool = {1.0}FC
  ks * MF
FCtrs:
 {1.0}FC = {1.0}FN
  k1n * FC - k2n * FN
MFdeg:
 {1.0}MF = $pool
  vm * MF / (km + MF)
MFtrn:
 $pool = {1.0}MF
  (vs + amp * ((1 + tanh(2 * (time - 24 * floor(time / 24) - dawn))) * (1 - tanh(2 * (time - 24 * floor(time / 24) - dusk))) / 4)) * pow(ki, n) / (pow(ki, n) + pow(FN + FNp, n))


#parameters
amp = 0.0
dawn = 6.0
dusk = 18.0
k1n = 0.222636680929471
k1np = 0.272306464006464
k2n = 0.331484503209686
k2np = 0.295420749525813
ki = 5.04543346939346
km = 0.0846004096489894
ks = 0.313846476998244
ksp = 0.294840169149965
n = 6.3958
vd = 0.161111487362275
vdp = 0.139750313979272
vm = 0.885376326739544
vs = 1.2236333742524
cytoplasm = 1.0
nucleus = 1.0


#initial values
FC = 2.46246334204771
FCp = 2.7123114824279
FN = 1.84400040515923
FNp = 2.74224951500791
MF = 0.725579308537909


#assignment rules
!F Tot_FRQ = FC + FCp + FN + FNp