#adlung1



#reactions
v1:
 {1.0}BaF3Epo = $pool
  Epodegradation * BaF3Epo
v18:
 $pool = {1.0}RpSHIP1
  kSHIP1 * (SHIP1tBaF3 - RpSHIP1) * (pEpoR - RpSHIP1)
v19:
 {1.0}RpSHIP1 = $pool
  lSHIP1 * RpSHIP1
v2:
 $pool = {1.0}pEpoR
  EpoRactivation1 / (1 + phos2 / Ki1) * (EpoRBaF3 - pEpoR * (1 + kPI3K / ((1 + kPI3K) * 1 + kPI3K * kPI3Kras * 1) + kPI3K * kPI3Kras / ((1 + kPI3K) * 1 + kPI3K * kPI3Kras * 1))) * (pow(BaF3Epo, nb) / (pow(ksatb, nb) + pow(BaF3Epo, nb)))
v20:
 $pool = {1.0}PIP3
  vPIP3 * PI45P2BaF3 * kPI3K / ((1 + kPI3K) * 1 + kPI3K * kPI3Kras * 1) * pEpoR
v21:
 $pool = {1.0}PIP3
  vPIP3 * main1 * PI45P2BaF3 * kPI3K * kPI3Kras / ((1 + kPI3K) * 1 + kPI3K * kPI3Kras * 1) * pEpoR
v22:
 {1.0}PIP3 = $pool
  kshbasal * PIP3 * (SHIP1tBaF3 - RpSHIP1)
v23:
 {1.0}PIP3 = $pool
  vPI45P2 * PIP3 * PTENBaF3
v24:
 {1.0}PIP3 = $pool
  vPI34P2 * PIP3 * RpSHIP1
v25:
 {1.0}PIP3 = {1.0}PIP3PDK1
  kPDK1 * PIP3 * (PDK1tBaF3 - PIP3PDK1)
v26:
 {1.0}PIP3PDK1 = {1.0}PIP3
  lPDK1 * PIP3PDK1
v27:
 {1.0}PIP3 = {1.0}PIP3Akt
  kAkt * PIP3 * (AkttBaF3 - PIP3Akt - pAkt)
v28:
 {1.0}PIP3Akt = {1.0}PIP3
  lAkt * PIP3Akt
v29:
 {1.0}PIP3Akt = $pool
  vpAkt * PIP3Akt * PIP3PDK1
v3:
 $pool = {1.0}actPhosphatase
  Phosphataseactivation1 * (PhosphatasetBaF3 - actPhosphatase) * pEpoR
v30:
 $pool = {1.0}PIP3
  vpAkt * PIP3Akt * PIP3PDK1
v31:
 $pool = {1.0}pAkt
  vpAkt * PIP3Akt * PIP3PDK1 / (1 + kaktinb * Aktin)
v32:
 {1.0}pAkt = $pool
  dpAkt * pAkt
v33:
 $pool = {1.0}PIP3
  vPIP3basal * PI45P2BaF3 * (PI3KtBaF3 - kPI3K / ((1 + kPI3K) * 1 + kPI3K * kPI3Kras * 1) * pEpoR - kPI3K * kPI3Kras / ((1 + kPI3K) * 1 + kPI3K * kPI3Kras * 1) * pEpoR)
v34:
 $pool = {1.0}PIP3
  vPIP3basal * main1 * PI45P2BaF3
v35:
 $pool = {1.0}pSos
  kpes * (Sostb - pSos - RpSos) * ppErk
v36:
 {1.0}pSos = $pool
  kdpes * pSos
v37:
 $pool = {1.0}RpSos
  kSos * (Sostb - RpSos - pSos) * (pEpoR - RpSos)
v38:
 {1.0}RpSos = $pool
  lSos * RpSos
v39:
 {1.0}RpSos = {1.0}pSos
  kpes * RpSos * ppErk
v4:
 {1.0}actPhosphatase = $pool
  Phosphatasedeactivation1 * actPhosphatase
v40:
 $pool = {1.0}GTPRas
  kbackRas * (RastBaF3 - GTPRas - kPI3K * kPI3Kras / ((1 + kPI3K) * 1 + kPI3K * kPI3Kras * 1) * pEpoR)
v41:
 $pool = {1.0}GTPRas
  kaRas * RpSos * (RastBaF3 - GTPRas - kPI3K * kPI3Kras / ((1 + kPI3K) * 1 + kPI3K * kPI3Kras * 1) * pEpoR)
v42:
 {1.0}GTPRas = $pool
  kdRas * GTPRas
v43:
 $pool = {1.0}pRaf
  kpRaf * (GTPRas + kPI3K * kPI3Kras / ((1 + kPI3K) * 1 + kPI3K * kPI3Kras * 1) * pEpoR) * (RaftBaF3 - pRaf)
v44:
 {1.0}pRaf = $pool
  lpRaf * pRaf
v45:
 $pool = {1.0}pMek
  kpMek * pRaf * (MektBaF3 - pMek - ppMek)
v46:
 {1.0}pMek = $pool
  lpMek * pMek
v47:
 {1.0}pMek = {1.0}ppMek
  kppMek * pRaf * pMek
v48:
 {1.0}ppMek = {1.0}pMek
  lppMek * ppMek
v49:
 $pool = {1.0}pErk
  kpErk * ppMek * (ErktBaF3 - pErk - ppErk)
v5:
 {1.0}pEpoR = $pool
  pEpoRdeactivation1 * pEpoR * actPhosphatase
v50:
 {1.0}pErk = $pool
  lpErk * bDUSP3 * pErk
v51:
 {1.0}pErk = {1.0}ppErk
  kppErk * ppMek * pErk / (1 + kmekinb * Mekin)
v52:
 {1.0}ppErk = {1.0}pErk
  lppErk * bDUSP3 * ppErk
v53:
 $pool = {1.0}Intperk
  ppErk
v54:
 $pool = {1.0}Intpakt
  pAkt
v55:
 $pool = {1.0}mTORa
  kvpakt * pAkt * (mTORt - mTORa - TORC1 - TORC2)
v56:
 $pool = {1.0}mTORa
  kvppErk * RSKa / (1 + kinrskb * RSKin) * (mTORt - mTORa - TORC1 - TORC2)
v57:
 {1.0}mTORa = $pool
  kdactm * mTORa
v58:
 {1.0}mTORa = {1.0}TORC1
  kst1 / (1 + kinrapab * Rapamycin) * (mTORa - TORC1 - TORC2) * (Raptorb - TORC1)
v59:
 {1.0}TORC1 = {1.0}mTORa
  kdt1 * TORC1
v6:
 $pool = {1.0}phos2
  Phosact1 * pow(pEpoR, n1) / (pow(Ji1, n1) + pow(pEpoR, n1))
v60:
 {1.0}TORC1 = $pool
  kdactm * TORC1
v61:
 {1.0}mTORa = {1.0}TORC2
  kst2 * (mTORa - TORC2 - TORC1) * (Rictorb - TORC2)
v62:
 {1.0}TORC2 = {1.0}mTORa
  kdt2 * TORC2
v63:
 {1.0}TORC2 = $pool
  kdactm * TORC2
v64:
 $pool = {1.0}RSKa
  karsk / (1 + kinrskb * RSKin) * (RSKtb - RSKa) * ppErk
v65:
 {1.0}RSKa = $pool
  kdrsk * RSKa
v66:
 $pool = {1.0}pS6
  kas6t * TORC1 * (S6tBaF3 - pS6)
v67:
 $pool = {1.0}pS6
  kas6r * RSKa * (S6tBaF3 - pS6)
v68:
 $pool = {1.0}pS6
  kbacks6 * (S6tBaF3 - pS6)
v69:
 {1.0}pS6 = $pool
  kdphoss6 * pS6
v7:
 {1.0}phos2 = $pool
  Phosdeact1 * phos2
v71:
 $pool = {1.0}IntpS6
  pS6
v73:
 $pool = {1.0}Proliferation
  pbk2correct * ((cycleaktinb * pAkt + cyclemekinb * ppErk) / maxccb) + pboffcorrect / 60


#parameters
Aktin = 0.0
EpoRactivation1 = 0.140964
Epodegradation = 0.0173287
Ji1 = 0.220505
Ki1 = 0.0272784
Mekin = 0.0
Phosact1 = 341.317
Phosdeact1 = 1e-05
Phosphataseactivation1 = 144.425
Phosphatasedeactivation1 = 1e-05
RSKin = 0.0
Rapamycin = 0.0
Sostb = 4.46181e-05
bDUSP3 = 96.7699
cycleaktinb = 0.967522
cyclemekinb = 0.127989
dpAkt = 3.4739
initAkttBaF3 = 0.51
initErktBaF3 = 2.964
initMektBaF3 = 4.38
initPDK1tBaF3 = 0.763
initPI3KtBaF3 = 0.0124
initPI45P2BaF3 = 10.0
initPTENBaF3 = 0.1074
initRSKtb = 3.0306
initRaftBaF3 = 3.886
initRastBaF3 = 9.531
initRpSHIP1 = 0.0
initS6tBaF3 = 2.59
initSHIP1tBaF3 = 0.0842
initTORC1 = 0.0
kAkt = 9984.96
kPDK1 = 9439.5
kPI3K = 0.000104741
kPI3Kras = 10000.0
kSHIP1 = 0.0745937
kSos = 59.4049
kaRas = 2981.53
kaktinb = 1.866219406
karsk = 1.26435
kas6r = 0.000533083
kas6t = 2180.44
kbackRas = 0.0103384
kbacks6 = 1.00003e-05
kdRas = 0.540777
kdactm = 0.0617342
kdpes = 0.0270729
kdphoss6 = 0.328006
kdrsk = 0.677229
kdt1 = 1588.13
kdt2 = 1e-05
kinrapab = 2.778901656
kinrskb = 0.552054462
kmekinb = 3.224998336
kpErk = 9999.08
kpMek = 9774.26
kpRaf = 1.17959
kpes = 0.583255
kppErk = 9999.96
kppMek = 9999.77
ksatb = 0.0024577
kshbasal = 1e-05
kst1 = 20.0908
kst2 = 0.0619403
kvpakt = 0.000174996
kvppErk = 0.000282332
lAkt = 10.1007
lPDK1 = 2355.54
lSHIP1 = 0.000650697
lSos = 0.39616
lpErk = 177.216
lpMek = 0.0414313
lpRaf = 2549.69
lppErk = 3.2516
lppMek = 1336.21
main1 = 0.000224787
maxccb = 0.99
n1 = 4.0
nb = 2.03939
pEpoRdeactivation1 = 3.20488
pbk2correct = 0.485791
pboffcorrect = 0.470245
vPI34P2 = 9996.96
vPI45P2 = 84.5564
vPIP3 = 10000.0
vPIP3basal = 0.104429
vpAkt = 6.90774
cyt = 1.0


#initial values
AkttBaF3 = 0.51
BaF3Epo = 0.002
EpoRBaF3 = 0.01862
ErktBaF3 = 2.964
GTPRas = 0.17879248230044018
IntpS6 = 0.0
Intpakt = 0.0
Intperk = 0.0
MektBaF3 = 4.38
PDK1tBaF3 = 0.763
PI3KtBaF3 = 0.0124
PI45P2BaF3 = 10
PIP3 = 0.0014259096174786036
PIP3Akt = 0.296484152509495
PIP3PDK1 = 0.004335113669239996
PTENBaF3 = 0.1074
PhosphatasetBaF3 = 0.0415
Proliferation = 0.0
RSKa = 0.06843365642650674
RSKtb = 3.0306
RaftBaF3 = 3.886
Raptorb = 0.1
RastBaF3 = 9.531
Rictorb = 1.2329
RpSHIP1 = 0
RpSos = 0.0
S6tBaF3 = 2.59
SHIP1tBaF3 = 0.0842
TORC1 = 0
TORC2 = 0.0
actPhosphatase = 0.0
mTORa = 0.0
mTORt = 0.1
pAkt = 0.0025557633868391167
pEpoR = 0.0
pErk = 0.017777899006355905
pMek = 4.312731699382008
pRaf = 0.00032128827441495054
pS6 = 0.0003669720694067856
pSos = 0.0
phos2 = 0.0
ppErk = 0.005858735624008178
ppMek = 0.010369614478384468


#assignment rules
!F pS6plot = 412.8573*pS6