Info! This is a derivative of the model smallbone11
Info! This is a derivative of the model levchenko1
v_1
Sr = Sext
v_2
v_2
Sext = Sint
v_3
Sext = p
v_4
v_4
Sint = Xint
v_5
Xint = Biom
v_6
Biom = p
Note that constraints are not enforced in simulations. It remains the responsibility of the user to verify that simulation results satisfy these constraints.
Control of specific growth rate in Saccharomyces cerevisiae.
Microbiology (Reading, Engl.) 2009;
155
:
1699-1707
- PubMed: 19359324
- DOI: 10.1099/mic.0.023119-0
- ISSN: 1350-0872
- URL: http://ncbi.nlm.nih.gov/pubmed/19359324
Abstract
In this contribution we resolve the long-standing dispute whether or not the Monod constant (K(S)), describing the overall affinity of an organism for its growth-limiting substrate, can be related to the affinity of the transporter for that substrate (K(M)). We show how this can be done via the control of the transporter on the specific growth rate; they are identical if the transport step has full control. The analysis leads to the counter-intuitive result that the affinity of an organism for its substrate is expected to be higher than the affinity of the enzyme that facilitates its transport. Experimentally, we show this indeed to be the case for the yeast Saccharomyces cerevisiae, for which we determined a K(M) value for glucose more than two times higher than the K(S) value in glucose-limited chemostat cultures. Moreover, we calculated that at glucose concentrations of 0.03 and 0.29 mM, the transport step controls the specific growth rate at 78 and 49 %, respectively.
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