vPFvALD
f16bpPF = dhapPF + gapPF
vPFvATPASE
atpPF = adpPF
vPFvENO
p2gPF = pepPF
vPFvG3PDH
nadhPF + dhapPF = g3pPF + nadPF
vPFvGAPDH
gapPF + nadPF = nadhPF + b13pgPF
vPFvGLCtr
glcEX = glcPF
vPFvGLYtr
g3pPF = glyEX
vPFvHK
glcPF + atpPF = g6pPF + adpPF
vPFvLACtr
lacPF = lacEX
vPFvLDH
nadhPF + pyrPF = lacPF + nadPF
vPFvPFK
atpPF + f6pPF = adpPF + f16bpPF
vPFvPGI
g6pPF = f6pPF
vPFvPGK
adpPF + b13pgPF = atpPF + p3gPF
vPFvPGM
p3gPF = p2gPF
vPFvPK
adpPF + pepPF = atpPF + pyrPF
vPFvPYRtr
pyrPF = pyrEX
vPFvTPI
dhapPF = gapPF
Assignment rules
hPF = pHConversionFactor/10.0^phPF
hEX = pHConversionFactor/10.0^phEX
Function definitions
Note that constraints are not enforced in simulations. It remains the responsibility of the user to verify that simulation results satisfy these constraints.
Construction and validation of a detailed kinetic model of glycolysis in Plasmodium falciparum.
FEBS J. 2015;
282
(8):
1481-1511
- PubMed: 25693925
- DOI: 10.1111/febs.13237
- ISSN: 1742-4658
- URL: http://ncbi.nlm.nih.gov/pubmed/25693925
Abstract
UNLABELLED: The enzymes in the Embden-Meyerhof-Parnas pathway of Plasmodium falciparum trophozoites were kinetically characterized and their integrated activities analyzed in a mathematical model. For validation of the model, we compared model predictions for steady-state fluxes and metabolite concentrations of the hexose phosphates with experimental values for intact parasites. The model, which is completely based on kinetic parameters that were measured for the individual enzymes, gives an accurate prediction of the steady-state fluxes and intermediate concentrations. This is the first detailed kinetic model for glucose metabolism in P. falciparum, one of the most prolific malaria-causing protozoa, and the high predictive power of the model makes it a strong tool for future drug target identification studies. The modelling workflow is transparent and reproducible, and completely documented in the SEEK platform, where all experimental data and model files are available for download.
DATABASE: The mathematical models described in the present study have been submitted to the JWS Online Cellular Systems Modelling Database (http://jjj.bio.vu.nl/database/penkler). The investigation and complete experimental data set is available on SEEK (10.15490/seek.1.
INVESTIGATION: 56).
DATABASE: The mathematical models described in the present study have been submitted to the JWS Online Cellular Systems Modelling Database (http://jjj.bio.vu.nl/database/penkler). The investigation and complete experimental data set is available on SEEK (10.15490/seek.1.
INVESTIGATION: 56).
No additional notes are available for this model.