v_1
source = CYCD1cdk4
v_10
v_10
source = MPF
v_11
MPF = sink
v_2
v_2
CYCD1cdk4 = sink
v_3
v_3
source = CYCEcdk2
v_4
v_4
CYCEcdk2 = sink
v_5
v_5
source = Rbhypo + unphosRb
v_6
Rbhypo + freeE2F = RbE2F
v_7
v_7
RbE2F + unphosRb = freeE2F
v_8
v_8
source = MPF
v_9
v_9
source = MPF
Note that constraints are not enforced in simulations. It remains the responsibility of the user to verify that simulation results satisfy these constraints.
A mathematical model of the regulation of the G1 phase of Rb+/+ and Rb-/- mouse embryonic fibroblasts and an osteosarcoma cell line.
Cell Prolif. 1997;
30
:
171-194
- PubMed: 9375029
- ISSN: 0960-7722
- URL: https://ncbi.nlm.nih.gov/pubmed/9375029
Abstract
A mathematical model integrating the roles of cyclin D, cdk4, cyclin E, cdk2, E2F and RB in control of the G1 phase of the cell cycle is described. Experimental results described with murine embryo fibroblasts (MEFs), either Rb+/+ or Rb-/-, and with the RB-deficient osteosarcoma cell line, Saos-2, served as the basis for the formulation of this mathematical model. A model employing the known interactions of these six proteins does not reproduce the experimental observations described in the MEFs. The appropriate modelling of G1 requires the inclusion of a sensing mechanism which adjusts the activity of cyclin E/cdk2 in response to both RB concentration and growth factors. Incorporation of this sensing mechanism into the model allows it to reproduce most of the experimental results observed in Saos-2 cells, Rb-/- MEFS, and Rb+/+ MEFs. The model also makes specific predictions which have not been tested experimentally.
No additional notes are available for this model.