$\frac{\mathrm{CDK2cycA}\times 599990\times 1E6}{43200}+\frac{\mathrm{CDK2cycA\_star\_}\times 599990\times 1E6}{43200}+\mathrm{basal\_fluorescence}$ Morris et al 2002 JBC 277(26): 23847--23853 kf and kb values not published kobs2 = 2.5 +- 0.1 /sec kf=0.813/sec and kb=0.557/sec used simulation $\mathrm{kf}\mathrm{CDK2cycA}\mathrm{geometry}-\mathrm{kb}\mathrm{CDK2cycA\_star\_}\mathrm{geometry}$ kf = 19/sec/uM and kb = 25/sec Morris et al 2002 JBC 277(26): 23847--23853 Table pp.23849 $\mathrm{kf}\mathrm{Cdk2}\mathrm{CyclinA}\mathrm{geometry}-\mathrm{kb}\mathrm{CDK2cycA}\mathrm{geometry}$