Etot=E1+E2 is constant.

species_5 species_2 compartment_0 species_0 species_2 k1 compartment_0 k2 species_1 species_3

During the initial phase, t smaller than T1 (T1=1), the whole amount of protein is allocated to the first reaction(E1=Etot, E2=0). At the beginning of the second phase the concentration E2 undergoes an abrupt switch from zero to a finite vaule whereas the concentration E1 is descreased by the same extent.

time 1

at time=1, E1=0.4

0.4